Antigen: human CMKLR1 amino acids 358–371 (NERTSMNERETGML) · Host: rabbit · Application(s): FC, ICC, and WB · Chemokine-Like Receptor 1 (CMKLR1) is a G protein-coupled receptor relevant to the cellular chemotaxis of dendritic cells and macrophages.¹ This receptor is also expressed in brain, liver, lung, and kidney tissues.^1,2 Chemerin, or TIG2, has been identified as the natural ligand for this receptor.³ Resolvin E1 has also been identified as a ligand for CMKLR1; acting to dampen cellular responses to inflammation.^1,4,5 Chemerin is an 18 kDa protein that plays a role in immunity, inflammation, chemotaxis, and has been identified as an adipokine.^6,7,2 Human CMKLR1 shares 80% sequence homology with its mouse ortholog.³ The human receptor exists in two isoforms, differing only by two amino acids due to a premature stop codon. Isoform one has 363 amino acids and an expected molecular weight of 42.3 kDa. Cayman’s CMKLR1 polyclonal antibody recognizes the C-terminal region of the protein and cell permeabilization is recommended prior to whole cell staining.

1 Arita, M., Bianchini, F., Aliberti, J., et al. Sterochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1. J Exp Med 201(5) 713-722 (2005).

2 Takahashi, M., Takahashi, Y., Takahashi, K., et al. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett 582 573-578 (2008).

3 Wittamer, V., Grégoire, F., Robberecht, P., et al. The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with low nanomolar potency. J Biol Chem 279(11) 9956-9962 (2004).

4 Arita, M., Ohira, T., Sun, Y., et al. Resolvin E1 selectively interacts with leukotriene B₄ receptor BLT₁ and ChemR23 to regulate inflammation. J Immunol 178 3912-3917 (2007).

5 Cash, J.L., Hart, R., Russ, A., et al. Synthetic chemerin-derived peptides suppress inflammation through ChemR23. J Environ Monit 205(4) 767-775 (2008).

6 Roh, S., Song, S., Choi, K., et al. Chemerin-a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun 362 1013-1018 (2007).

7 Campbell, E.L., Louis, N.A., Tomassetti, S.E., et al. Resolvin E1 promotes mucosal surface clearance of neutrophils: a new paradigm for inflammatory resolution. FASEB J 21 3162-3170 (2007).

Roh, S., Song, S., Choi, K., et al. Chemerin-a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun 362 1013-1018 (2007).

Arita, M., Bianchini, F., Aliberti, J., et al. Sterochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1. J Exp Med 201(5) 713-722 (2005).

Takahashi, M., Takahashi, Y., Takahashi, K., et al. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett 582 573-578 (2008).

Cash, J.L., Hart, R., Russ, A., et al. Synthetic chemerin-derived peptides suppress inflammation through ChemR23. J Environ Monit 205(4) 767-775 (2008).

Campbell, E.L., Louis, N.A., Tomassetti, S.E., et al. Resolvin E1 promotes mucosal surface clearance of neutrophils: a new paradigm for inflammatory resolution. FASEB J 21 3162-3170 (2007).

Wittamer, V., Grégoire, F., Robberecht, P., et al. The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with low nanomolar potency. J Biol Chem 279(11) 9956-9962 (2004).

Arita, M., Ohira, T., Sun, Y., et al. Resolvin E1 selectively interacts with leukotriene B₄ receptor BLT₁ and ChemR23 to regulate inflammation. J Immunol 178 3912-3917 (2007).

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