Antigen: human AdPLA₂ amino acids 147-162 (MSLIGVMFSRNKRQKQ) · Host: rabbit · Application(s): WB · AdPLA₂ is the first member of Group XVI phospholipase A₂ (PLA₂). The PLA₂ superfamily consists of multiple enzymes that catalyze the hydrolysis of fatty acids from the sn-2 position in phospholipids. This pH- and calcium-dependent PL is highly expressed in adipose tissue and is associated with adipocyte differentiation and lipolysis. It has been implicated as a major player in the development of obesity.^1,2 Human AdPLA₂ is a 162 amino acid protein with a calculated molecular weight of 18 kDa. It contains a transmembrane region and shares 70-75% sequence similarity with the mouse and rat proteins, respectively. Cayman’s antibody was raised against the C-terminal region of the human protein.

1 Jaworski, K., Ahmadian, M., Duncan, R.E., et al. AdPLA ablation increases lipolysis and prevents obesity induced by high-fat feeding or leptin deficiency. Nat Med 15(2) 159-168 (2009).

2 Duncan, R.E., Sarkadi-Nagy, E., Jaworski, K., et al. Identification and functional characterization of adipose-specific phospholipase A₂ (AdPLA). J Biol Chem 283(37) 25428-25436 (2008).

Jaworski, K., Ahmadian, M., Duncan, R.E., et al. AdPLA ablation increases lipolysis and prevents obesity induced by high-fat feeding or leptin deficiency. Nat Med 15(2) 159-168 (2009).

Duncan, R.E., Sarkadi-Nagy, E., Jaworski, K., et al. Identification and functional characterization of adipose-specific phospholipase A₂ (AdPLA). J Biol Chem 283(37) 25428-25436 (2008).