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Griffithsin

Cayman Chemical Item Number 10340

GRFT

Griffithsin

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Description

Source: Recombinant N-terminal His-tagged GRFT, purified from E. coli · Mr: 14.6 kDa · Griffithsin (GRFT), is a lectin isolated from Griffithsia sp. with anti-HIV activity at subnanomolar concentrations.1 GRFT activity is glycosylation-dependent and acts by binding to glycoproteins gp41, gp120, and gp160 on the viral envelope, blocking the virus from binding to CD4 receptor-expressing cells in the host. It also prevents cell fusion between infected and uninfected cells, further inhibiting the spread of HIV in the body. GRFT functions as a homodimer, with each monomer containing three carbohydrate binding sites.1,2 GRFT unique anti-viral activity is of interest for use in microbicide production to stop transmission of HIV through sexual contact. It has also been shown to have inhibitory effects with SARS-related coronavirus; where GRFT binding to the SARS-CoV S protein can prevent viral entry and reduce viral load in succeeding rounds of infection.3

1 Mori, T., O'Keefe, B.R., Sowder, R.C., et al. Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp.. J Biol Chem 280(10) 9345-9353 (2005).

2 Ziólkowska, N.E., O'Keefe, B.R., Mori, T., et al. Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding. Structure 14 1127-1135 (2006).

3 O'Keefe, B.R., Giomarelli, B., Barnard, D.L., et al. Broad spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J Virol (2009).

Synonyms
  • GRFT
Formulation 50 mM sodium phosphate pH 7.2, containing100 mM sodium chloride, 2.5 mM DTT, and 20% glycerol
Purity ≥95%
Stability 9 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Giomarelli, B., Schumacher, K.M., Taylor, T.E., et al. Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture. Protein Expr Purif 47 194-202 (2006).

Mori, T., O'Keefe, B.R., Sowder, R.C., et al. Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp.. J Biol Chem 280(10) 9345-9353 (2005).
Size Price Quantity Subtotal
25 µg $92.00 $0.00
50 µg $175.00 $0.00
100 µg $331.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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