Cytokeratin Monoclonal FITC Antibody (Clone C-11)

Cayman Chemical Item Number 10349

pan-Cytokeratin

Cytokeratin Monoclonal FITC Antibody (Clone C-11)

Description

Host: mouse · Application(s): FC and IF · This pan-cytokeratin antibody detects cytokeratin isoforms 4, 5, 6, 8,10,13, and 18.¹ Cytokeratins make up a large family of proteins classified as intermediate filament polypeptides.^1,2 They are subdivided into type I (acidic) and type II (basic/neutral) proteins.³ Under normal conditions, these proteins are found in epithelial cells as type I/type II pairs.^1,4 These pairs provide cellular support as well as contributing to more dynamic processes. Cytokeratins undergo extensive post translational modifications, such as phosphorylation, and are differentially expressed in normal and cancer cells.^4,5

1 Bártek, J., Vojtêek, B., Staková, Z., et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164 215-224 (1991).

2 Magin, T.M., Vijayaraj, P., and Leube, R.E. Structural and regulatory functions of keratins. Exp Cell Res 313 2021-2032 (2007).

3 Gu, L., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr Opin Cell Biol 19 13-23 (2007).

4 Ditzel, H.J., Strik, M.C.M., Larsen, M.K., et al. Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. J Biol Chem 277(24) 21712-21722 (2002).

5 Plowman, J.E. The proteomics of keratin proteins. J Chromatogr B 849 181-189 (2007).

Synonyms

pan-Cytokeratin

Formulation

100 μg of fluorescein-labeled IgG1 in 500 μl PBS, pH 7.2, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human Cytokeratin 4	+
Human Cytokeratin 5	+
Human Cytokeratin 6	+
Human Cytokeratin 8	+
Human Cytokeratin 10	+
Human Cytokeratin 13	+
Human Cytokeratin 18	+

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Background Reading

Ditzel, H.J., Strik, M.C.M., Larsen, M.K., et al. Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. J Biol Chem 277(24) 21712-21722 (2002).

Plowman, J.E. The proteomics of keratin proteins. J Chromatogr B 849 181-189 (2007).

Magin, T.M., Vijayaraj, P., and Leube, R.E. Structural and regulatory functions of keratins. Exp Cell Res 313 2021-2032 (2007).

Gu, L., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr Opin Cell Biol 19 13-23 (2007).

Bártek, J., Vojtêek, B., Staková, Z., et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164 215-224 (1991).

Show all 5 Hide all but first 3

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