Antigen: human EP₂ receptor C-terminal amino acids 335-358 · Host: rabbit · Application(s): FC and ICC · Peptide sequence: SLRTQDATQTSCSTQSDASKQADL · This polyclonal antibody is labeled with R-Phycoerythrin (PE) and can be used for immunofluorescent labeling of cellular EP₂ receptor following permeabilization. R-PE absorption maxima occurs at 565>540>498 nm and the emission maximum occurs at 578 nm, however standard excitation by 488 nm lasers is sufficient to generate signal detected in the PE channel of flow cytometers. The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^1,2 Binding of PGE₂ to the EP₂ receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.^3,4 Pharmacologically, EP₂ receptors mediate relaxation of smooth muscle and are distinguished from EP₄ receptors by their sensitivity to the EP₂ receptor selective agonist butaprost.^1,2,3 The human EP₂ receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000.³ mRNA for the EP₂ receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.^3,4,5

1 Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

2 Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Prostanoid and leukotriene receptors: A progress report from the IUPHAR working parties on classification and nomenclature. Adv Prostaglandin Thromboxane Leukot Res 23 283-285 (1995).

3 Narko, K., Saukkonen, K., Ketola, I., et al. Regulated expression of prostaglandin E₂ receptors EP₂ and EP₄ in human ovarian granulosa-luteal cells . J Clin Endocrinol Metab 86(4) 1765-1768 (2001).

4 Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54 713-725 (1997).

5 Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP₂ subtype. Mol Pharmacol 46 213-220 (1994).

Cosme, R., Lublin, D., Takafuji, V., et al. Prostanoids in human colonic mucosa: Effects of inflammation on PGE₂ receptor expression . Hum Immunol 61 684-696 (2000).

Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Prostanoid and leukotriene receptors: A progress report from the IUPHAR working parties on classification and nomenclature. Adv Prostaglandin Thromboxane Leukot Res 23 283-285 (1995).

Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

Narko, K., Saukkonen, K., Ketola, I., et al. Regulated expression of prostaglandin E₂ receptors EP₂ and EP₄ in human ovarian granulosa-luteal cells . J Clin Endocrinol Metab 86(4) 1765-1768 (2001).

Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54 713-725 (1997).

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