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BLT1 Receptor Polyclonal Antibody

Cayman Chemical Item Number 120114

Leukotriene B4 Receptor 1; LTB4 Receptor 1; BLTR1

BLT1 Receptor Polyclonal Antibody

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Description

Antigen: human BLT1 receptor amino acids 331-352 (ALEPGPSESLTASSPLKLNELN) · Host: rabbit · Application(s): WB, flow cytometry, ICC, and IHC · The leukotriene B4 receptor 1 (BLT1 receptor), cloned from HL-60 human leukemia cells, has 352 amino acids and seven putative membrane-spanning domains.1 The primary structure of the receptor is identical to that of a putative purinoceptor, P2Y7, which binds to micromolar concentrations of ATP.2 Northern blotting reveals that the BLT1 receptor is highly expressed in leukocytes, U937 cells, and to a much lower extent in spleen and thymus.1 Sheep lung membranes have also been identified as a rich source for receptor isolation and purification.3 A second LTB4 receptor, BLT2, has recently been cloned and characterized.4,5,6 This antibody was made against a peptide from the C-terminus of the BLT1 receptor, which is located on the intracellular side of the plasma membrane. Therefore, when performing studies on whole cells, permeabilization of the cells is required for the antibody to enter the cytosol. For flow cytometry and confocal microscopy analysis of the receptor, the antibody can be diluted approximately 1:2,000; use at a dilution of approximately 1:1,000 for Western blot analysis.

1 Yokomizo, T., Izumi, T., Chang, K., et al. A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis. Nature 387 620-624 (1997).

2 Akbar, G.K.M., Dasari, V.R., Webb, T.E., et al. Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells. J Biol Chem 271 18363-18367 (1996).

3 Votta, B., Keefer, J., and Mong, S. Characterization of the soluble leukotriene B4 receptor from sheep lung membranes. Biochem J 270 213-218 (1990).

4 Yokomizo, T., Kato, K., Terawaki, K., et al. A second leukotriene B4 receptor, BLT2: A new therapeutic target in inflammation and immunological disorders. J Exp Med 193 421-431 (2000).

5 Kamohara, M., Takasaki, J., Matsumoto, M., et al. Molecular cloning and characterization of another leukotriene B4 receptor. J Biol Chem 275 27000-27004 (2000).

6 Wang, S., Gustafson, E., Pang, L., et al. A novel hepatointestinal leukotriene B4 receptor. J Biol Chem 275 40686-40694 (2000).

Synonyms
  • Leukotriene B4 Receptor 1
  • LTB4 Receptor 1
  • BLTR1
Formulation Peptide affinity-purified IgG
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Human BLT1 receptor +
Bovine BLT1 receptor +
Mouse BLT1 receptor -

Background Reading

Akbar, G.K.M., Dasari, V.R., Webb, T.E., et al. Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells. J Biol Chem 271 18363-18367 (1996).

Votta, B., Keefer, J., and Mong, S. Characterization of the soluble leukotriene B4 receptor from sheep lung membranes. Biochem J 270 213-218 (1990).

Kamohara, M., Takasaki, J., Matsumoto, M., et al. Molecular cloning and characterization of another leukotriene B4 receptor. J Biol Chem 275 27000-27004 (2000).

Wang, S., Gustafson, E., Pang, L., et al. A novel hepatointestinal leukotriene B4 receptor. J Biol Chem 275 40686-40694 (2000).

Yokomizo, T., Kato, K., Terawaki, K., et al. A second leukotriene B4 receptor, BLT2: A new therapeutic target in inflammation and immunological disorders. J Exp Med 193 421-431 (2000).

Yokomizo, T., Izumi, T., Chang, K., et al. A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis. Nature 387 620-624 (1997).

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Size Price Quantity Subtotal
500 µl $225.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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BLT1 Receptor Polyclonal Antiserum
BLT2 Receptor Polyclonal Antibody
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