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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

(−)-Blebbistatin

Cayman Chemical Item Number 13013

(S)-Blebbistatin (CAS 856925-71-8)

(-)-Blebbistatin (CAS 856925-71-8)

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Description

(−)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.1,2 It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50 = 0.5-5.0 μM), while poorly inhibiting smooth muscle myosin (IC50 = 80 μM).3 Through these effects, blebbistatin blocks apoptosis-related bleb formation, directed cell migration and cytokinesis in vertebrate cells. Blebbistatin is inactivated by UV light,4 which may be particularly important in fluorescent cell imaging applications.

1 Straight, A.F., Cheung, A., Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743-1747 (2003).

2 Kovács, M., Tóth, J., Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34) 35557-35563 (2004).

3 Limouze, J., Straight, A.F., Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 25 337-341 (2004).

4 Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem Biophys Res Commun 320 1020-1025 (2004).

Synonyms
  • (S)-Blebbistatin
Formal Name 1,​2,​3,​3a-​tetrahydro-​3aS-​hydroxy-​6-​methyl-​1-​phenyl-​4H-​Pyrrolo[2,​3-​b]quinolin-​4-​one
CAS Number 856925-71-8
Molecular Formula C18H16N2O2
Formula Weight 292.3
Formulation A crystalline solid
Purity ≥98%
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
Copy SMILES to clipboard
CC1=CC2C(C=C1)​N=C1N(CC[C@@]​1(O)​C2=O)​c1ccccc1

Background Reading

Limouze, J., Straight, A.F., Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 25 337-341 (2004).

Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem Biophys Res Commun 320 1020-1025 (2004).

Straight, A.F., Cheung, A., Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743-1747 (2003).

Kovács, M., Tóth, J., Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34) 35557-35563 (2004).

Harb, N., Archer, T.K., and Sato, N. The Rho-rock-myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells. PLoS One 3(8) (2008).

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(-)-Blebbistatin is available in the following screening library:

Size Price Quantity Subtotal
1 mg $23.00 $0.00
5 mg $104.00 $0.00
10 mg $184.00 $0.00
25 mg $406.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-11. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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