Antigen: human ApoAI amino acids 188-199 · Clone designation: CC3821C4 · Host: Mouse · Application(s): WB · ApoAI is a major protein component in high-density lipoproteins (HDLs). It acts as an acceptor for sequential transfers of phospholipids and free cholesterol from peripheral tissues and transports cholesterol to the liver and other tissues for excretion and steroidogenesis.¹ Serum ApoAI levels are inversely related to the risk of developing atherosclerosis.² Loss-of-function mutations are causes of diseases such as HDL deficiency type 1 (or Tangier disease) and type 2 (familial hypoalphalipoproteinemia), and systemic non-neuropathic amyloidosis.^3,4 Liver and small intestine are two main sources of the protein. ApoAI is comprised of a single polypeptide chain of 243 amino acid residues with an estimated molecular weight of 28 kDa. Cayman’s ApoAI Monoclonal Antibody detects the protein from diluted human plasma (≤10 µg total protein) by western blotting. Western blotting of recombinant ApoAI samples suggest a detection limit of 5 ng.

1 Ajees, A.A., Anantharamaiah, G.M., Mishra, V.K., et al. Crystal structure of human apolipoprotein A-I: Insights into its protective effect against cardiovascular diseases. Proc Natl Acad Sci USA 103(7) 2126-2131 (2006).

2 Haas, M.J., Reinacher, D., Li, J.P., et al. Regulation of apoA1 gene expression with acidosis: Requirement for a transcriptional repressor. J Mol Endocrinol 27 43-57 (2001).

3 Tall, A.R., and Wang, N. Tangier disease as a test of the reverse cholesterol transport hypothesis. J Clin Invest 106(10) 1205-1207 (2000).

4 Cheung, M.C., Mendez, A.J., Wolf, A.C., et al. Characterization of apolipoprotein A-I- and A-II-containing lipoproteins in a new case of high density lipoprotein deficiency resembling tangier disease and their effects on intracellular cholesterol efflux. J Clin Invest 91 522-529 (1993).

Haas, M.J., Reinacher, D., Li, J.P., et al. Regulation of apoA1 gene expression with acidosis: Requirement for a transcriptional repressor. J Mol Endocrinol 27 43-57 (2001).

Tall, A.R., and Wang, N. Tangier disease as a test of the reverse cholesterol transport hypothesis. J Clin Invest 106(10) 1205-1207 (2000).

Cheung, M.C., Mendez, A.J., Wolf, A.C., et al. Characterization of apolipoprotein A-I- and A-II-containing lipoproteins in a new case of high density lipoprotein deficiency resembling tangier disease and their effects on intracellular cholesterol efflux. J Clin Invest 91 522-529 (1993).

Ajees, A.A., Anantharamaiah, G.M., Mishra, V.K., et al. Crystal structure of human apolipoprotein A-I: Insights into its protective effect against cardiovascular diseases. Proc Natl Acad Sci USA 103(7) 2126-2131 (2006).

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