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ThioFluor 623

Cayman Chemical Item Number 13083

Fluorescent Thiol Probe (CAS 1004324-99-5)

ThioFluor 623 (CAS 1004324-99-5)

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Description

The rapid, selective, and sensitive sensing of thiols is important in diverse areas of research. This thiofluor 623 responds upon exposure to thiols with an increase in fluorescence intensity of up to 120-fold.1 The response is selective for thiols and occurs in aqueous media.1 In the absence of thiols, the probe is essentially non-fluorescent; thiols cause cleavage of the probe, generating a fluorophore with an absorption maximum of 563 nm and emission at 623 nm.1 The fluorescence quantum yield of the cleaved product, generated in response to thiols, increases in more viscous media, suggesting ideal performance in biological systems and applicability to single-molecule or 2-photon sensing schemes. The thiofluor 623 is cell-permeable and reacts selectively with intracellular thiols.1 The pseudo-first order rate constant (kobs) depends on substrate (e.g., 2.1 x 10−3 s−1 for cysteine, 2.0 x 10−5 s−1 for human serum albumin).1

1 Bouffard, J., Kim, Y., Swager, T.M., et al. A highly selective fluorescent probe for thiol bioimaging. Org Lett 10(1) 37-40 (2008).

Synonyms
  • Fluorescent Thiol Probe
Formal Name N-​[4-​[(1E)-​2-​[4-​cyano-​5-​(dicyanomethylene)-​2,​5-​dihydro-​2,​2-​dimethyl-​3-​furanyl]ethenyl]phenyl]-​N-​[2-​[2-​(2-​methoxyethoxy)ethoxy]ethyl]2,​4-​dinitro-​benzenesulfonamide
CAS Number 1004324-99-5
Molecular Formula C31H30N6O10S
Formula Weight 678.7
Formulation A crystalline solid
Purity ≥98%
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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COCCOCCOCCN(c1ccc(cc1)​\C=C/C1=C(CN)​C(=C(CN)​CN)​OC1(C)​C)​S(=O)​(=O)​c1ccc(cc1[N]​(=O)​O)​[N]​(=O)​O

Background Reading

Bouffard, J., Kim, Y., Swager, T.M., et al. A highly selective fluorescent probe for thiol bioimaging. Org Lett 10(1) 37-40 (2008).
Size Price Quantity Subtotal
5 mg $138.00 $0.00
10 mg $262.00 $0.00
50 mg $1,104.00 $0.00
100 mg $1,932.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-05-26. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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