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(±)-Blebbistatin

Cayman Chemical Item Number 13186

(CAS 674289-55-5)

(±)-Blebbistatin (CAS 674289-55-5)

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Description

(±)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.1,2 It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50 = 0.5-5.0 μM), while poorly inhibiting smooth muscle myosin (IC50 = 80 μM).3 Through these effects, blebbistatin blocks apoptosis-related bleb formation, directed cell migration and cytokinesis in vertebrate cells. Blebbistatin is inactivated by UV light,4 which may be particularly important in fluorescent cell imaging applications. Purified enantiomers of Blebbistatin are also available from Cayman ((−)-Blebbistatin - Catalog No. 13013; (+)-Blebbistatin - Catalog No. 13165).

1 Straight, A.F., Cheung, A., Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743-1747 (2003).

2 Kovács, M., Tóth, J., Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34) 35557-35563 (2004).

3 Limouze, J., Straight, A.F., Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 25 337-341 (2004).

4 Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem Biophys Res Commun 320 1020-1025 (2004).

Formal Name 1,​2,​3,​3a-​tetrahydro-​3a-​hydroxy-​6-​methyl-​1-​phenyl-​4H-​pyrrolo[2,​3-​b]quinolin-​4-​one
CAS Number 674289-55-5
Molecular Formula C18H16N2O2
Formula Weight 292.3
Formulation A crystalline solid
Purity ≥98%
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
Copy SMILES to clipboard
Cc1ccc2N=C3N(CCC3(O)​C(=O)​c2c1)​c1ccccc1

Background Reading

Straight, A.F., Cheung, A., Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743-1747 (2003).

Limouze, J., Straight, A.F., Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 25 337-341 (2004).

Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem Biophys Res Commun 320 1020-1025 (2004).

Kovács, M., Tóth, J., Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34) 35557-35563 (2004).

Lucas-Lopez, C., Patterson, S., Blum, T., et al. Absolute stereochemical assignment and fluorescence tuning of the small molecule tool, (−)-blebbistatin. Eur J Org Chem 1736-1740 (2005).

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(±)-Blebbistatin is available in the following screening library:

Size Price Quantity Subtotal
1 mg $13.00 $0.00
5 mg $59.00 $0.00
10 mg $104.00 $0.00
25 mg $228.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

Related Products

(−)-Blebbistatin
(+)-Blebbistatin

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