TRPC4 Calcium Channel Monoclonal Antibody (Clone S77-15)

Cayman Chemical Item Number 13719

Transient Receptor Potential Cation Channel

Description

Antigen: synthetic peptide amino acids 930-947 of rat TRPC4 · Clone designation: S77-15 · Host: Mouse · Isotype: IgG2b · Application(s): WB, IP, and ICC · Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient.¹ They are present in the membranes that surround all biological cells and their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage-gated ion channels, ligand-gated, other gating systems, and finally those that are classified differently, having more exotic characteristics. The first are voltage-gated ion channels which open and close in response to membrane potential. These are then seperated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels, and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels and transient receptor potential channels.² Transient receptor potential cation channel, subfamily C, member 4 (TRPC4), is a human gene encoding a protein of the same name. They are expressed in smooth muscle and endothelial cells where they regulate membrane potential and calcium influx. TRPC4 is activated by G(q)/phospholipase C-coupled receptors, but the underlying mechanism remains elusive.³ Studies suggest TRPC4 contributes to axonal regeneration after nerve injury.⁴

1 Hille, B. Ion Channels of Excitable Membranes. 3rd (2001).

2 . What are ion channels? (2004).

3 Otsuguro, K., Tang, J., Xiao, R., et al. Isoform-specific inhibition of TRPC4 channel by phosphatidylinositol 4,5-bisphosphate. J Biol Chem 283(15) 10026-10036 (2008).

4 Wu, D., Huang, W., Richardson, P.M., et al. TRPC4 in rat dorsal root ganglion neurons is increased after nerve injury and is necessary for neurite outgrowth. J Biol Chem 283(1) 416-426 (2008).

Synonyms

Transient Receptor Potential Cation Channel

Formulation

100 µg of protein G-purified IgG in 100 µl PBS, pH 7.4, containing 50% glycerol and 0.09% sodium azide

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human TRPC4	+
Mouse TRPC4	+
Rat TRPC4	+

Background Reading

Hille, B. Ion Channels of Excitable Membranes. 3rd (2001).

. What are ion channels? (2004).

Otsuguro, K., Tang, J., Xiao, R., et al. Isoform-specific inhibition of TRPC4 channel by phosphatidylinositol 4,5-bisphosphate. J Biol Chem 283(15) 10026-10036 (2008).

Wu, D., Huang, W., Richardson, P.M., et al. TRPC4 in rat dorsal root ganglion neurons is increased after nerve injury and is necessary for neurite outgrowth. J Biol Chem 283(1) 416-426 (2008).

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Subtotal

100 µg

$290.00

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