Acetyl Lysine Polyclonal Antibody HRP Conjugate
Cayman Chemical Item Number 13726
Description
Antigen:
acetylated KLH
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Host:
rabbit
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Application(s):
WB, IP, ELISA, and IF
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Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of lysine involves acetylation.1 The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs).2 Protein post-translational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progession, apoptosis, DNA repair, and cytoskeletal orgnization.3 The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.4,5
1
Yang, X. Multisite protein modification and intramolecular signaling. Oncogene 24 1653-1662 (2005).
2
Hassig, C.A., and Schreiber, S.L. Nuclear histone acetylases and deacetylases and transcriptional regulation: HATs off to HDACs. Curr Opin Chem Biol 1(3) 300-308 (1997).
3
Yang, X. Lysine acetylation and the bromodomain: A new partnership for signaling. BioEssays 26(10) 1076-1087 (2004).
4
Hughes, R.E. Polyglutamine disease: Acetyltransferases awry. Curr Biol 12(4) R141-R143 (2002).
5
Vigushin, D.M., and Coombes, R.C. Targeted histone deacetylase inhibition for cancer therapy. Curr Cancer Drug Targets 4 205-218 (2004).
| Formulation |
400 µl (0.25 mg/ml) of rabbit immunoglobulin in PBS containing 0.01% sodium azide |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Room temperature
in continental US; may vary elsewhere
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| Specificity |
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Background Reading
Hughes, R.E. Polyglutamine disease: Acetyltransferases awry. Curr Biol 12(4) R141-R143 (2002).
Vigushin, D.M., and Coombes, R.C. Targeted histone deacetylase inhibition for cancer therapy. Curr Cancer Drug Targets 4 205-218 (2004).
Yang, X. Lysine acetylation and the bromodomain: A new partnership for signaling. BioEssays 26(10) 1076-1087 (2004).
Hassig, C.A., and Schreiber, S.L. Nuclear histone acetylases and deacetylases and transcriptional regulation: HATs off to HDACs. Curr Opin Chem Biol 1(3) 300-308 (1997).
Yang, X. Multisite protein modification and intramolecular signaling. Oncogene 24 1653-1662 (2005).
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Pricing updated 2012-05-26.
Prices are subject to change without notice.
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Warning This product is not for human or veterinary use.
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.
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