8-iso PGE₂ is one of several isoprostanes produced from arachidonic acid during lipid peroxidation.¹ It is a potent renal vasoconstrictor in the rat.^1,2 8-iso PGE₂ inhibits U-46619 or I-BOP-induced platelet aggregation with IC₅₀ values of 0.5 and 5 µM, respectively.³ When infused into the renal artery of the rat at a concentration of 4 µg/kg/min, 8-iso PGE₂ decreases the GFR and renal plasma flow by 80% without affecting blood pressure.¹

1 Morrow, J.D., Minton, T.A., Mukundan, C.R., et al. Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes. J Biol Chem 269 4317-4326 (1994).

2 Hoffman, S.W., Moore, S., and Ellis, E.F. Isoprostanes: Free radical-generated prostaglandins with constrictor effects on cerebral arterioles. Stroke 28 844-84 (1997).

3 Longmire, A.W., Roberts, L.J., and Morrow, J.D. Actions of the E₂-isoprostane, 8-iso-PGE₂, on the platelet thromboxane/endoperoxide receptor in humans and rats: Additional evidence for the existence of a unique isoprostane receptor. Prostaglandins 48 247-256 (1994).

Morrow, J.D., Minton, T.A., Mukundan, C.R., et al. Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes. J Biol Chem 269 4317-4326 (1994).

Hoffman, S.W., Moore, S., and Ellis, E.F. Isoprostanes: Free radical-generated prostaglandins with constrictor effects on cerebral arterioles. Stroke 28 844-84 (1997).

Longmire, A.W., Roberts, L.J., and Morrow, J.D. Actions of the E₂-isoprostane, 8-iso-PGE₂, on the platelet thromboxane/endoperoxide receptor in humans and rats: Additional evidence for the existence of a unique isoprostane receptor. Prostaglandins 48 247-256 (1994).

8-iso Prostaglandin E2 is available in the following screening libraries:

• Prostaglandin Screening Library II (96-Well)
• Bio-active Lipid I Screening Library (96-Well)