Antigen: purified ovine COX-1 · Host: rabbit · Application(s): WB and IHC · Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H₂ COX-1 is constitutively expressed in almost all animal tissues and is involved in the homeostatic role of eicosanoids.^1,2 Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.³ COX-1 and -2 are 70 and 72 kDa proteins, respectively. Both cyclooxygenases have been cloned from a variety of species including human, mouse, rat, and sheep.^4,5 COX-1 isoform at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%.

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Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).

Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).

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