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COX-1 Monoclonal Antibody (Clone CX111)

Cayman Chemical Item Number 160110

PGHS-1; Prostaglandin H Synthase 1; Cyclooxygenase 1

COX-1 Monoclonal Antibody (Clone CX111)

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Description

Antigen: purified ovine COX-1 · Clone designation: CX111 · Host: mouse · Isotype: IgG2b · Application(s): WB and IHC · COX-1 is responsible for the production of prostaglandins essential for the normal function of many organs including stomach and kidney.1 Although COX-1 is generally described as constitutively expressed, this is actually an oversimplification. COX-1 expression is regulated developmentally and in response to a variety of other stimuli.2,3,4,5 COX-1 has a molecular weight of 70,000 and is expressed in nearly all tissues of the body.1,3,6,7

1 Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

2 Brannon, T.S., North, A.J., Wells, L.B., et al. Prostacyclin synthesis in ovine pulmonary artery is developmentally regulated by changes in cyclooxygenase-1 gene expression. J Clin Invest 93 2230-2235 (1994).

3 Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

4 Samet, J.M., Fasano, M.B., Fonteh, A.N., et al. Selective induction of prostaglandin G/H synthase I by stem cell factor and dexamethasone in mast cells. J Biol Chem 270 8044-8049 (1995).

5 Hla, T., and Maciag, T. Cyclooxygenase gene expression is down-regulated by heparin-binding (acidic fibroblast) growth factor-1 in human endothelial cells. J Biol Chem 266 24059-24063 (1991).

6 DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

7 DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Synonyms
  • PGHS-1
  • Prostaglandin H Synthase 1
  • Cyclooxygenase 1
Formulation Lyophilized IgG
Stability 3 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Hla, T., and Maciag, T. Cyclooxygenase gene expression is down-regulated by heparin-binding (acidic fibroblast) growth factor-1 in human endothelial cells. J Biol Chem 266 24059-24063 (1991).

Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Créminon, C., Frobert, Y., Habib, A., et al. Immunological studies of human constitutive cyclooxygenase (COX-1) using enzyme immunometric assay. Biochim Biophys Acta 1254 333-340 (1995).

Samet, J.M., Fasano, M.B., Fonteh, A.N., et al. Selective induction of prostaglandin G/H synthase I by stem cell factor and dexamethasone in mast cells. J Biol Chem 270 8044-8049 (1995).

Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

Macchia, L., Di Paola, R., Guerrese, M., et al. Expression of prostaglandin endoperoxide H synthase 1 and 2 in human placenta at term. Biochem Biophys Res Commun 233 496-501 (1997).

Pang, L., and Knox, A.J. Effect of interleukin-1β, tumour necrosis factor-α and interferon-γ on the induction of cyclo-oxygenase-2 in cultured human airway smooth muscle cells. Br J Pharmacol 121 579-587 (1997).

Brannon, T.S., North, A.J., Wells, L.B., et al. Prostacyclin synthesis in ovine pulmonary artery is developmentally regulated by changes in cyclooxygenase-1 gene expression. J Clin Invest 93 2230-2235 (1994).

Uotila, P., Valve, E., Martikainen, P., et al. Increased expression of cyclooxygenase-2 and nitric oxide synthase-2 in human prostate cancer. Urol Res 29 25-28 (2001).

Smith, T.J., Jennings, T.A., Sciaky, D., et al. Prostaglandin-endoperoxide H synthase-2 expression in human thyroid epithelium. J Biol Chem 274(22) 15622-15632 (1999).

Duffy, D.M., and Stouffer, R.L. The ovulatory gonadotrophin surge stimulates cyclooxygenase expression and prostaglandin production by the monkey follicle. Mol Hum Reprod 7(8) 731-739 (2001).

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This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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