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COX-1 Monoclonal FITC Antibody (Clone CX111)

Cayman Chemical Item Number 160111

PGHS-1; Prostaglandin H Synthase 1; Cyclooxygenase 1

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Description

Antigen: purified ovine COX-1 · Clone designation: CX111 · Host: mouse · Isotype: IgG2b · Application(s): FC and IF · This product is derived by labeling the COX-1 monoclonal antibody (Item No. 160110) with fluorescein. COX-1 catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. COX-1 is constitutively expressed in almost all animal tissues and is involved in the homeostatic role of eicosanoids.1,2 COX-1 is a 70 kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep.3,4,5,6 COX-1 from these species are approximately 90% identical at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%.

1 Smith, W.L., and Marnett, L.J. Prostaglandin endoperoxide synthase: Structure and catalysis. Biochim Biophys Acta 1083 1-17 (1991).

2 Marnett, L.J., and Maddipati, K.R. Prostaglandin H synthase. 293-334 (1991).

3 Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

4 DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

5 Kitzler, J., Hill, E., Hardman, R., et al. Analysis and quantitation of splicing variants of the TPA-inducible PGHS-1 mRNA in rat tracheal epithelial cells. Arch Biochem Biophys 316 856-863 (1995).

6 DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Synonyms
  • PGHS-1
  • Prostaglandin H Synthase 1
  • Cyclooxygenase 1
Formulation 250 µg IgG in 500 µl PBS, pH 7.2, with 50% glycerol, 0.5 mg/ml BSA and 0.02% NaN3
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

Smith, W.L., and Marnett, L.J. Prostaglandin endoperoxide synthase: Structure and catalysis. Biochim Biophys Acta 1083 1-17 (1991).

DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

Marnett, L.J., and Maddipati, K.R. Prostaglandin H synthase. 293-334 (1991).

DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Kitzler, J., Hill, E., Hardman, R., et al. Analysis and quantitation of splicing variants of the TPA-inducible PGHS-1 mRNA in rat tracheal epithelial cells. Arch Biochem Biophys 316 856-863 (1995).

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Size Price Quantity Subtotal
250 µg $246.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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COX-2 Monoclonal FITC Antibody (Clone CX229)
COX-2 (mouse) Polyclonal FITC Antibody Exclusive
COX Polyclonal Antibody
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