Antigen: purified ovine COX-1 · Clone designation: CX111 · Host: mouse · Isotype: IgG2b · Application(s): FC and IF · This product is derived by labeling the COX-1 monoclonal antibody (Item No. 160110) with phycoerythrin. COX-1 is responsible for the production of prostaglandins essential for the normal function of many organs including stomach and kidney.¹ Although COX-1 is generally described as constitutively expressed, this is actually an oversimplification. COX-1 expression is regulated developmentally and in response to a variety of other stimuli.^2,3,4,5 COX-1 has a molecular weight of 70,000 and is expressed in nearly all tissues of the body.^1,3,6,7

1 Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

2 Brannon, T.S., North, A.J., Wells, L.B., et al. Prostacyclin synthesis in ovine pulmonary artery is developmentally regulated by changes in cyclooxygenase-1 gene expression. J Clin Invest 93 2230-2235 (1994).

3 Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

4 Samet, J.M., Fasano, M.B., Fonteh, A.N., et al. Selective induction of prostaglandin G/H synthase I by stem cell factor and dexamethasone in mast cells. J Biol Chem 270 8044-8049 (1995).

5 Hla, T., and Maciag, T. Cyclooxygenase gene expression is down-regulated by heparin-binding (acidic fibroblast) growth factor-1 in human endothelial cells. J Biol Chem 266 24059-24063 (1991).

6 DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

7 DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Hla, T., and Maciag, T. Cyclooxygenase gene expression is down-regulated by heparin-binding (acidic fibroblast) growth factor-1 in human endothelial cells. J Biol Chem 266 24059-24063 (1991).

Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).

DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., et al. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem 265 5192-5198 (1990).

DeWitt, D.L., and Smith, W.L. Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc Natl Acad Sci USA 85 1412-1416 (1988).

Samet, J.M., Fasano, M.B., Fonteh, A.N., et al. Selective induction of prostaglandin G/H synthase I by stem cell factor and dexamethasone in mast cells. J Biol Chem 270 8044-8049 (1995).

Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

Brannon, T.S., North, A.J., Wells, L.B., et al. Prostacyclin synthesis in ovine pulmonary artery is developmentally regulated by changes in cyclooxygenase-1 gene expression. J Clin Invest 93 2230-2235 (1994).

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