Prostaglandin E Synthase (cytosolic) Polyclonal Antibody

Cayman Chemical Item Number 160150

cPGES; cPGE Synthase

Description

Antigen: human cPGE synthase amino acids 58-67 (CIDPNDSKHK) · Host: rabbit · Application(s): WB and IHC · Prostaglandin E Synthase (PGE Synthase) catalyzes the isomerization of PGH₂ to PGE₂. Cytosolic and microsomal PGE synthase enzymes have been cloned and characterized. Cytosolic PGE synthase (cPGE synthase) is a glutathione-dependent enzyme with a predicted size of 18.6 kDa (23 kDa on SDS-PAGE). The enzyme is expressed in a wide variety of tissues and cells, the levels of which are unaffected by treatment with IL-1β and TNFα.¹ However, enzyme expression increases approximately 3-fold in rat brain following LPS treatment.¹ Microsomal PGE synthase is a 16 kDa protein expressed in a variety of tissues including prostate, testes, and small intestine, as well as in A549 and HeLa cells.² In contrast to cPGE synthase, mPGE synthase protein expression is increased in A549 cells following treatment with IL-1β.² The two enzymes show <10% homology at the amino acid level.

1 Tanioka, T., Nakatani, Y., Semmyo, N., et al. Molecular identification of cytosolic prostaglandin E₂ synthase that is functionally coupled with cyclooxygenase-1 immediate prostaglandin E₂ biosynthesis. J Biol Chem 275 32775-32782 (2000).

2 Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).

Synonyms

cPGES
cPGE Synthase

Formulation

Peptide affinity-purified IgG in TBS containing 50% glycerol, 0.1% BSIgG, lyophilized

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human cPGES	+
Mouse cPGES	+
Ovine cPGES	+
Human recombinant mPGES	-

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Background Reading

Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).

Tanioka, T., Nakatani, Y., Semmyo, N., et al. Molecular identification of cytosolic prostaglandin E₂ synthase that is functionally coupled with cyclooxygenase-1 immediate prostaglandin E₂ biosynthesis. J Biol Chem 275 32775-32782 (2000).