Antigen: human and rat amino acids 130-149 (QHRRKELETRQKQYRWMEWN); The sequence is 95% homologous to 5-LO from mouse and hamster · Host: rabbit · Application(s): WB · 5-Lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid as well as its subsequent conversion to leukotriene A₄.¹ 5-LO is located in the cytosol of quiescent cells. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.^2,3 5-LO has a molecular mass of approximately 78 kDa. The enzyme is predominately expressed in peripheral blood leukocytes and also in eosinophils, mast cells, epithelial cells, and alveolar macrophages isolated from lung.⁴

1 Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes leukotriene A₄ synthesis from arachidonic acid. Proc Natl Acad Sci USA 81 689-693 (1984).

2 Pouliot, M., McDonald, P.P., Krump, E., et al. Colocalization of cytosolic phospholipase A₂, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils. Eur J Biochem 238 250-258 (1996).

3 Brock, T.G., McNish, R.W., Bailie, M.B., et al. Rapid import of cytosolic 5-lipoxygenase into the nucleus of neutrophils after in vivo recruitment and in vitro adherence. J Biol Chem 272 8276-8280 (1997).

4 Komatsu, N., Natsui, K., Ueda, N., et al. Immunohistochemical study on arachidonate 5-lipoxygenase in porcine leukocytes and other tissues. J Histochem Cytochem 39 655-662 (1991).

Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes leukotriene A₄ synthesis from arachidonic acid. Proc Natl Acad Sci USA 81 689-693 (1984).

Brock, T.G., McNish, R.W., Bailie, M.B., et al. Rapid import of cytosolic 5-lipoxygenase into the nucleus of neutrophils after in vivo recruitment and in vitro adherence. J Biol Chem 272 8276-8280 (1997).

Chen, N., Restivo, A., and Reiss, C.S. Selective inhibition of COX-2 is beneficial to mice infected intranasally with VSV. Prostaglandins Other Lipid Mediat 67 143-155 (2002).

Pouliot, M., McDonald, P.P., Krump, E., et al. Colocalization of cytosolic phospholipase A₂, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils. Eur J Biochem 238 250-258 (1996).

Komatsu, N., Natsui, K., Ueda, N., et al. Immunohistochemical study on arachidonate 5-lipoxygenase in porcine leukocytes and other tissues. J Histochem Cytochem 39 655-662 (1991).

Uz, T., Dwivedi, Y., Savani, P.D., et al. Glucocorticoids stimulate inflammatory 5-lipoxygenase gene expression and protein translocation in the brain. J Neurochem 73 693-699 (1999).

Mehrabian, M., Allayee, H., Wong, J., et al. Identification of 5-lipoxygenase as a major gene contributing to atherosclerosis susceptibility in mice. Circ Res 91 120-126 (2002).

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