Antigen: hamster iPLA₂ amino acids 557-576 (PRFNQNINLKPPTQPADQLV) · Host: rabbit · Application(s): WB; other applications not tested · Calcium-independent phospholipase A₂ (iPLA₂) is one of the most recently characterized isoforms in the growing list of the phospholipase A₂ enzyme family.¹ iPLA₂ has been purified or cloned from CHO cells and mouse P388D1 macrophage-like cells and is the major isoform of phospholipase A₂ found in A-10 smooth muscle cells.^2,3,4,5,6,7 The enzyme has a molecular weight of about 80-85 kDa and is classified as a type VI PLA₂.1-5 The enzyme from CHO cells is approximately 95% and 90% homologous to mouse and human iPLA₂, respectively.^2,3

1 Dennis, E.A. The growing phospholipase A₂ superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).

2 Tang, J., Kriz, R.W., Wolfman, N., et al. A novel cytosolic calcium-independent phospholipase A₂ contains eight ankyrin motifs. J Biol Chem 272 8567-8575 (1997).

3 Balboa, M.A., Balsinde, J., Jones, S.S., et al. Identity between the Ca2+-independent phospholipase A₂ enzymes from P388D1 macrophages and Chinese hamster ovary cells. J Biol Chem 272 8576-8580 (1997).

4 Wolf, M.J., and Gross, R.W. Expression, purification, and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A₂. J Biol Chem 271 30879-30885 (1996).

5 Ackermann, E.J., Kempner, E.S., and Dennis, E.A. Ca2+-independent cytosolic phospholipase A₂ from macrophage-like P388D1 cells. J Biol Chem 269 9227-9233 (1994).

6 Wolf, M.J., Wang, J., Turk, J., et al. Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A₂. J Biol Chem 272 1522-1526 (1997).

7 Wolf, M.J., and Gross, R.W. The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A₂. Implications for cardiac cycle-dependent alterations in phospholipolysis. J Biol Chem 271 20989-20992 (1996).

Ackermann, E.J., Kempner, E.S., and Dennis, E.A. Ca2+-independent cytosolic phospholipase A₂ from macrophage-like P388D1 cells. J Biol Chem 269 9227-9233 (1994).

Wolf, M.J., and Gross, R.W. Expression, purification, and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A₂. J Biol Chem 271 30879-30885 (1996).

Balboa, M.A., Balsinde, J., Jones, S.S., et al. Identity between the Ca2+-independent phospholipase A₂ enzymes from P388D1 macrophages and Chinese hamster ovary cells. J Biol Chem 272 8576-8580 (1997).

Wolf, M.J., and Gross, R.W. The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A₂. Implications for cardiac cycle-dependent alterations in phospholipolysis. J Biol Chem 271 20989-20992 (1996).

Wolf, M.J., Wang, J., Turk, J., et al. Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A₂. J Biol Chem 272 1522-1526 (1997).

Dennis, E.A. The growing phospholipase A₂ superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).

Tang, J., Kriz, R.W., Wolfman, N., et al. A novel cytosolic calcium-independent phospholipase A₂ contains eight ankyrin motifs. J Biol Chem 272 8567-8575 (1997).

Atsumi, G., Murakami, M., Kojima, K., et al. Distinct roles of two intracellular phospholipase A₂s in fatty acid release in the cell death pathway. Proteolytic fragment of type IVA cytosolic phospholipase A2α inhibits stimulus-induced arachidonate release, whereas that of type VI Ca2+-independent phospholipase A₂ augments spontaneous fatty acid release. J Biol Chem 275 18248-18258 (2000).

Isenovic, E., and LaPointe, M.C. Role of Ca2+-independent phospholipase A₂ in the regulation of inducible nitric oxide synthase in cardiac myocytes. Hypertension 35 249-254 (2000).

Roshak, A.K., Capper, E.A., Stevenson, C., et al. Human calcium-independent phospholipase A₂ mediates lymphocyte proliferation. J Biol Chem 275 35692-35698 (2000).

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