Antigen: human PAF receptor amino acids 1-17 (MEPHDSSHMDSEFRYTL) · Host: rabbit · Application(s): FC, ICC, and WB · PAF is a potent phospholipid mediator which exerts diverse biological actions by interaction with a G protein-coupled PAF receptor. The PAF receptor has been cloned from a number of species including human, rat, and guinea pig and is characterized as a 7-transmembrane receptor which induces phosphoinositol turnover through G-protein coupling.^1,2,3,4,5 Northern blot analysis reveals that the receptor is expressed in leukocytes, placenta, lung, spleen, small intestine, kidney, liver, and brain.^3,4 In leukocyte cell populations the receptor is found on platelets, monocytes, neutrophils, and B-cells, whereas resting T-cells and natural killer cell lines do not express the PAF receptor.⁶ Human monocytes treated with INF-γ have a 2-6 fold increase in PAF receptor expression compared to untreated cells.⁷ PAF receptor is detected on immunoblot at 48 kDa.⁸

1 Nakamura, M., Honda, Z., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. J Biol Chem 266(30) 20400-20405 (1991).

2 Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. J Biol Chem 267 9101-9106 (1992).

3 Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem Biophys Res Commun 180 105-111 (1991).

4 Bito, H., Honda, Z., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur J Biochem 227 211-218 (1994).

5 Mutoh, H., Bito, H., Minami, M., et al. Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor. FEBS Lett 322 129-134 (1993).

6 Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc Natl Acad Sci USA 90 5818-5822 (1993).

7 Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J Immunol 152 5092-5099 (1994).

8 Marrache, A.M., Gobeil, F. Jr., Bernier, S.G., et al. Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptor. J Immunol 169(11) 6474-6481 (2002).

Mutoh, H., Bito, H., Minami, M., et al. Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor. FEBS Lett 322 129-134 (1993).

Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J Immunol 152 5092-5099 (1994).

Nakamura, M., Honda, Z., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. J Biol Chem 266(30) 20400-20405 (1991).

Marrache, A.M., Gobeil, F. Jr., Bernier, S.G., et al. Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptor. J Immunol 169(11) 6474-6481 (2002).

Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem Biophys Res Commun 180 105-111 (1991).

Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. J Biol Chem 267 9101-9106 (1992).

Bito, H., Honda, Z., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur J Biochem 227 211-218 (1994).

Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc Natl Acad Sci USA 90 5818-5822 (1993).

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