Antigen: bovine lung PGIS · Clone designation: isn-1 · Host: mouse · Isotype: IgG₁ · Application(s): IP and IHC; WB not recommended · PGI synthase (PGIS) catalyzes the conversion of PGH₂ to PGI₂ (prostacyclin). Human lung PGIS is a 56 kDa protein. The cloned bovine and human enzymes contain 500 amino acids and have a calculated molecular mass of 56,629 and 57,103, respectively.^1,2,3 There is 88% homology between human and bovine PGIS.

1 Miyata, A., Hara, S., Yokoyama, C., et al. Molecular cloning and expression of human prostacyclin synthase. Biochem Biophys Res Commun 200 1728-1734 (1994).

2 Pereira, B., Wu, K.K., and Wang, L. Molecular cloning and characterization of bovine prostacyclin synthase. Biochem Biophys Res Commun 203 59-66 (1994).

3 Hara, S., Miyata, A., Yokoyama, C., et al. Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem 269 19897-19903 (1994).

DeWitt, D.L., and Smith, W.L. Purification of prostacyclin synthase from bovine aorta by immunoaffinity chromatography. Evidence that the enzyme is a hemoprotein. J Biol Chem 258 3285-3293 (1983).

Hara, S., Miyata, A., Yokoyama, C., et al. Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem 269 19897-19903 (1994).

Miyata, A., Hara, S., Yokoyama, C., et al. Molecular cloning and expression of human prostacyclin synthase. Biochem Biophys Res Commun 200 1728-1734 (1994).

Pereira, B., Wu, K.K., and Wang, L. Molecular cloning and characterization of bovine prostacyclin synthase. Biochem Biophys Res Commun 203 59-66 (1994).

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