Antigen: human recombinant Nod2 residues 28-301 · Host: rabbit · Application(s): WB and IP; may not detect basal levels of Nod2 in monocytes · Nod2 is a member of a protein family of apoptosis regulators which includes Apaf-1 and Nod1/CARD4.¹ Nod2 contains two N-terminal caspase recruitment domains (CARDs), a nucleotide binding domain (NBD) and C-terminal leucine rich repeats (LRRs). Nod1/CARD4 is broadly expressed in tissues; however, Nod2 expression is limited to monocytes.^1,2 Both Nod1 and Nod2 activate NF-kB and act as intracellular receptors for bacterial lipopolysaccharides (LPS).^2,3,4,5 The LRRs of both Nod1 and Nod2 are essential for LPS-induced activation of NF-kB.⁴ A frameshift mutation in the NOD2 gene confers susceptibility to Crohn’s disease, possibly by causing truncation of the 10^th LRR resulting in a protein that is unresponsive to LPS.^6,7

1 Ogura, Y., Inohara, N., Benito, A., et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J Biol Chem 276 4812-4818 (2001).

2 Bertin, J., Nir, W., Fischer, C.M., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem 274 12955-12958 (1999).

3 Inohara, N., Koseki, T., Lin, J., et al. An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J Biol Chem 275 27823-27831 (2000).

4 Inohara, N., Ogura, Y., Chen, F.F., et al. Human NOD1 confers responsiveness to bacterial lipopolysaccharides. J Biol Chem 276 2551-2554 (2001).

5 Inohara, N., Koseki, T., del Peso, L., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J Biol Chem 274 14560-14567 (1999).

6 Ogura, Y., Bonen, D.K., Inohara, N., et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature 411 603-606 (2001).

7 Hugot, J., Chamaillard, M., Zouali, H., et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature 411 599-603 (2001).

Bertin, J., Nir, W., Fischer, C.M., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem 274 12955-12958 (1999).

Inohara, N., Koseki, T., del Peso, L., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J Biol Chem 274 14560-14567 (1999).

Ogura, Y., Inohara, N., Benito, A., et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J Biol Chem 276 4812-4818 (2001).

Hugot, J., Chamaillard, M., Zouali, H., et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature 411 599-603 (2001).

Inohara, N., Koseki, T., Lin, J., et al. An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J Biol Chem 275 27823-27831 (2000).

Inohara, N., Ogura, Y., Chen, F.F., et al. Human NOD1 confers responsiveness to bacterial lipopolysaccharides. J Biol Chem 276 2551-2554 (2001).

Ogura, Y., Bonen, D.K., Inohara, N., et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature 411 603-606 (2001).

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