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Antigen:
purified enzyme from mouse macrophages (RAW 264.7)
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Host:
rabbit
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Application(s):
WB, IP, and ICC
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Nitric Oxide Synthase (NOS) catalyzes the biosynthesis of nitric oxide from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS). iNOS is a soluble enzyme found in a variety of tissues including macrophages, hepatocytes, vascular smooth muscle cells, and chondrocytes.1,2 iNOS expression is increased by a variety of factors including LPS, IFN-γ, Il-1β, and TNF-α, whereas expression is deceased by dexamethasone.3,4,5,6,7 The enzyme has been cloned from several species including mouse, rat, and human with homology of at least 80% between these species.2 The calculated molecular weight of the protein from the deduced amino acid sequence is 130,000 - 131,000.1
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1
Knowles, R.G., Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).
2
Michel, T., Xie, Q., Nathan, C. Molecular biological analysis of nitric oxide synthases. (1996).
3
Lorsbach, R.B., Murphy, W.J., Lowenstein, C.J., et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-γ and lipopolysaccharide. J Biol Chem 268 1908-1913 (1993).
4
Balligand, J., Ungureanu-Longrois, D., Simmons, W.W., et al. Cytokine-inducible nitric oxide synthase (iNOS) expression in cardiac myocytes. Characterization and regulation of iNOS expression and detection of iNOS activity in single cardiac cyocytes in vivo. J Biol Chem 269 27580-27588 (1994).
5
Schmidt, H.H.H.W., Warner, T.D., Nakane, M., et al. Regulation and subcellular location of nitrogen oxide synthases in RAW264.7 macrophages. Mol Pharmacol 41 615-624 (1992).
6
Ogura, T., Esumi, H. Nitric oxide synthase expression in human neuroblastoma cell line induced by cytokines. Mol Neurosci 7 853-856 (1996).
7
Walker, G., Pfeilschifter, J., Kunz, D. Mechanism of suppression of inducible nitric-oxide synthase (iNOS) expression in interferon (IFN)-γ-stimulated RAW 264.7 cells by dexamethasone. Evidence for glucocorticoid-induced degradation of iNOS protein by calpain as a key step in post-transcriptional regulation. J Biol Chem 271 16679-16687 (1996).
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