Antigen: human nNOS amino acids 1422-1433 (ESKKDTDEVFSS) · Host: rabbit · Application(s): ICC, IHC, IP, and WB · Nitric oxide synthase (NOS) catalyzes the oxidation of arginine to nitric oxide (NO) and citrulline. Three distinct isoforms of NOS have been described having nomenclature based on the tissue source from which they were originally cloned. These three isoforms are neuronal/brain NOS (nNOS/bNOS/NOS-I), inducible NOS (iNOS/NOS-II), and endothelial NOS (eNOS/NOS-III).^1,2 nNOS is a soluble enzyme found in brain, the peripheral nervous system and skeletal muscle.^3,4 An alternately spliced form of nNOS (nNOS · ) containing a 34 amino acid insert has been identified in skeletal muscle.⁵ In neurons, protein-protein interactions with PSD95 and PSD93 via the PZD domain at the N-terminus of nNOS localizes the enzyme with NMDA receptors.^6,7 Although nNOS was originally thought to be constitutively expressed, abundant evidence suggests expression is regulated by a variety of conditions.⁸

1 Michel, T., Xie, Q., and Nathan, C. Molecular biological analysis of nitric oxide synthases. 161-175 (1996).

2 Michel, T., and Feron, O. Nitric oxide synthases: Which, where, how, and why? J Clin Invest 100 2146-2152 (1997).

3 Bredt, D.S., Hwang, P.M., Glatt, C.E., et al. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature 351 714-718 (1991).

4 Nakane, M., Schmidt, H.H.H.W., Pollock, J.S., et al. Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett 316 175-180 (1993).

5 Silvagno, F., Xia, H., and Bredt, D.S. Neuronal nitric-oxide synthase-μ, an alternatively spliced isoform expressed in differentiated skeletal muscle. J Biol Chem 271 11204-11208 (1996).

6 Brenman, J.E., Christopherson, K.S., Craven, S.E., et al. Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein. J Neurosci 16 7407-7415 (1996).

7 Christopherson, K.S., and Bredt, D.S. Nitric oxide in excitable tissues: Physiological roles and disease. J Clin Invest 100 2424-2429 (1997).

8 Wang, Y., Newton, D.C., and Marsden, P.A. Neuronal NOS: Gene structure, mRNA diversity, and functional relevance. Crit Rev Neurobiol 13 21-43 (1999).

Bredt, D.S., Hwang, P.M., Glatt, C.E., et al. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature 351 714-718 (1991).

Nakane, M., Schmidt, H.H.H.W., Pollock, J.S., et al. Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett 316 175-180 (1993).

Silvagno, F., Xia, H., and Bredt, D.S. Neuronal nitric-oxide synthase-μ, an alternatively spliced isoform expressed in differentiated skeletal muscle. J Biol Chem 271 11204-11208 (1996).

Michel, T., Xie, Q., and Nathan, C. Molecular biological analysis of nitric oxide synthases. 161-175 (1996).

Brenman, J.E., Christopherson, K.S., Craven, S.E., et al. Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein. J Neurosci 16 7407-7415 (1996).

Michel, T., and Feron, O. Nitric oxide synthases: Which, where, how, and why? J Clin Invest 100 2146-2152 (1997).

Wang, Y., Newton, D.C., and Marsden, P.A. Neuronal NOS: Gene structure, mRNA diversity, and functional relevance. Crit Rev Neurobiol 13 21-43 (1999).

Christopherson, K.S., and Bredt, D.S. Nitric oxide in excitable tissues: Physiological roles and disease. J Clin Invest 100 2424-2429 (1997).

Smith, F.S., and Titheradge, M.A. Detection of NOS isoforms by western-blot analysis. Methods Mol Biol 100 171-180 (1998).

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