See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Guanylate Cyclase α subunit (soluble) Polyclonal Antibody

Cayman Chemical Item Number 160895

sGC α1 subunit

See more

Description

Antigen: human guanylate cyclase α subunit amino acids 418-436 (EQARAQDGLKKRLGKLKAT) · Host: rabbit · Application(s): WB · Soluble guanylate cyclase is a heterodimeric enzyme, composed of α and β subunits, that synthesizes cGMP from GTP. The enzyme is activated by the binding of nitric oxide or carbon monoxide to the heme group of the enzyme.1 Chronic hypoxia upregulates soluble guanylate expression in rat lung.2 The α1 subunit contains 690-717 amino acids and has a molecular mass of 77-82 kDa.3,4,5 The cloned β1 subunit of guanylate cyclase from human, bovine, and rat sources contains 619 amino acids and has a molecular mass of approximately 70,000.3,6,7

1 Stone, J.R., and Marletta, M.A. Soluble guanylate cyclase from bovine lung: Activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states. Biochemistry 33 5636-5640 (1994).

2 Li, D., Zhou, N., and Johns, R.A. Soluble guanylate cyclase gene expression and localization in rat lung after exposure to hypoxia. Am J Physiol 277 841-847 (1999).

3 Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett 304 83-88 (1992).

4 Nakane, M., Arai, K., Saheki, S., et al. Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung. J Biol Chem 265 16841-16845 (1990).

5 Lee, M., and Bell, R.M. Supplementation of the phosphatidyl-L-serine requirement of protein kinase C with nonactivating phospholipids. Biochemistry 31 5176-5182 (1992).

6 Koesling, D., Herz, J., Gausepohl, H., et al. The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase. FEBS Lett 239 29-34 (1988).

7 Nakane, M., Saheki, S., Kuno, T., et al. Molecular cloning of a cDNA coding for 70 kilodalton subunit of soluble guanylate cyclase from rat lung. Biochem Biophys Res Commun 157 1139-1147 (1988).

Synonyms
  • sGC α1 subunit
Formulation 500 µg affinity-purified IgG in 500 µl TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Human guanylate cyclase α subunit +
Mouse guanylate cyclase α subunit +
Bovine guanylate cyclase α subunit +

Background Reading

Stone, J.R., and Marletta, M.A. Soluble guanylate cyclase from bovine lung: Activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states. Biochemistry 33 5636-5640 (1994).

Li, D., Zhou, N., and Johns, R.A. Soluble guanylate cyclase gene expression and localization in rat lung after exposure to hypoxia. Am J Physiol 277 841-847 (1999).

Nakane, M., Arai, K., Saheki, S., et al. Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung. J Biol Chem 265 16841-16845 (1990).

Nakane, M., Saheki, S., Kuno, T., et al. Molecular cloning of a cDNA coding for 70 kilodalton subunit of soluble guanylate cyclase from rat lung. Biochem Biophys Res Commun 157 1139-1147 (1988).

Koesling, D., Herz, J., Gausepohl, H., et al. The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase. FEBS Lett 239 29-34 (1988).

Koesling, D., Harteneck, C., Humbert, P., et al. The primary structure of the larger subunit of soluble guanylyl cyclase from bovine lung. Homology between the two subunits of the enzyme. FEBS Lett 266 128-132 (1990).

Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett 304 83-88 (1992).

Show all 7 Hide all but first 3
Size Price Quantity Subtotal
500 µl $161.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

Guanylate Cyclase α subunit (soluble) Blocking Peptide
Guanylate Cyclase β1 subunit (soluble) Polyclonal Antibody
LY83583
ODQ
YC-1
Show all 5 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?