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Antigen:
rat soluble guanylate cyclase β1 subunit amino acids 188-207 (EDFYEDLDRFEENGTQDSR; last D=E in human and bovine)
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Host:
rabbit
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Application(s):
WB and IHC
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Soluble guanylate cyclase is a heterodimeric enzyme, composed of a and b subunits, that catalyzes the synthesis of cGMP from GTP. The enzyme is activated by the binding of nitric oxide to the heme group of the enzyme.1 The cloned b1 subunit of guanylate cyclase from human, bovine, and rat sources contains 619 amino acids and has a molecular mass of approximately 70,000.2,3,4 The a1 subunit contains 690-717 amino acids and has a molecular mass of 77-82 kDa.3,5,6 This antibody will specifically recognize only the b1 subunit of soluble guanylate cyclase.
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1
Stone, J.R., Marletta, M.A. Soluble guanylate cyclase from bovine lung: Activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states. Biochemistry 33 5636-5640 (1994).
2
Nakane, M., Saheki, S., Kuno, T., et al. Molecular cloning of a cDNA coding for 70 kilodalton subunit of soluble guanylate cyclase from rat lung. Biochem Biophys Res Commun 157 1139-1147 (1988).
3
Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett 304 83-88 (1992).
4
Koesling, D., Herz, J., Gausepohl, H., et al. The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase. FEBS Lett 239 29-34 (1988).
5
Nakane, M., Arai, K., Saheki, S., et al. Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung. J Biol Chem 265 16841-16845 (1990).
6
Koesling, D., Harteneck, C., Humbert, P., et al. The primary structure of the larger subunit of soluble guanylyl cyclase from bovine lung. Homology between the two subunits of the enzyme. FEBS Lett 266 128-132 (1990).
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