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COX-2 (ovine) Electrophoresis Standard

Cayman Chemical Item Number 360120

Prostaglandin H Synthase 2; Cyclooxygenase 2; PGHS-2

See more	Cyclooxygenase (97) Eicosanoids (216) Electrophoresis Standard (5) Prostaglandins (513) Proteins (180)

Description

M_r: 72.00 · Cyclooxygenase (COX) catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H₂. Recent discoveries of the induction of cyclooxygenase by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.^1,2 COX-2 is a 72 kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep.^3,4,5,6

1 Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

2 Arakawa, T., Laneuville, O., Miller, C.A., et al. Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP₄ receptor gene. J Biol Chem 271 29569-29575 (1996).

3 Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).

4 Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).

5 Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).

6 Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).

Synonyms	Prostaglandin H Synthase 2 Cyclooxygenase 2 PGHS-2
Formulation	5 µg COX-2 standard in 50 µl 20 mM Tris-HCl, pH 8.0
Purity	>95%
Stability	1 year
Storage	-80°C
Shipping	Dry ice in continental US; may vary elsewhere

Background Reading

Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).

Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).

Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).

Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).

Arakawa, T., Laneuville, O., Miller, C.A., et al. Prostanoid receptors of murine NIH 3T3 and RAW 264.7 cells. Structure and expression of the murine prostaglandin EP₄ receptor gene. J Biol Chem 271 29569-29575 (1996).

Johnson, J.L., Wimsatt, J., Buckel, S.D., et al. Purification and characterization of prostaglandin H synthase-2 from sheep placental cotyledons. Arch Biochem Biophys 324 26-34 (1995).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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