sPLA2 (murine Type V) Blocking Peptide

sPLA₂ (murine Type V) Blocking Peptide

Cayman Chemical Item Number 360512

Secretory Phospholipase A₂

Description

Each vial contains 200 µg of lyophilized peptide derived from sPLA₂ (murine Type V) (amino acids 79-94; CAIRTQSYDYRYTNGL). This peptide was used as an antigen for production of Cayman’s sPLA₂ (murine Type V) polyclonal antibody (Catalog No. 160512) and should be used in conjunction with this antibody to block protein-antibody complex formation during analysis for sPLA₂ (type V).

Synonyms

Secretory Phospholipase A2

Formulation

A lyophilized peptide

Purity

>50%

Stability

2 years

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A₂ involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

Dennis, E.A. The growing phospholipase A₂ superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).

Cupillard, L., Koumanov, K., Mattéi, M., et al. Cloning, chromosomal mapping, and expression of a novel human secretory phospholipase A₂. J Biol Chem 272 15745-15752 (1997).

Bingham, C.O., Fijneman, R.J.A., Friend, D.S., et al. Low molecular weight group IIA and group V phospholipase A₂ enzymes have different intracellular locations in mouse bone marrow-derived mast cells. J Biol Chem 274 31476-31484 (1999).

Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning, expression and partial characterization of a novel rat phospholipase A₂. Biochim Biophys Acta 1215 115-120 (1994).

Oishi, H., Noto, T., Sasaki, H., et al. Thiolactomycin, a new antibiotic; Taxonomy of the producing organism, fermentation and biological properties. J Antibiot 35 391-395 (1982).

Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A₂ that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning and recombinant expression of a novel human low molecular weight Ca2+-dependent phospholipase A₂. J Biol Chem 269 2365-2368 (1994).

Tischfield, J.A., Xia, Y., Shih, D.M., et al. Low-molecular-weight, calcium-dependent phospholipase A₂ genes are linked and map to homologous chromosome regions in mouse and human. Genomics 32 328-333 (1996).

Bingham, C.O., Murakami, M., Fujishima, H., et al. A heparin-sensitive phospholipase A₂ and prostaglandin endoperoxide synthase-2 are functionally linked in the delayed phase of prostaglandin D₂ generation in mouse bone marrow-derived mast cells. J Biol Chem 271 25936-25944 (1996).