Prostaglandin E2 Express EIA Kit

Prostaglandin E₂ Express EIA Kit

Cayman Chemical Item Number 500141

PGE₂ Express EIA Kit; Prostaglandin E₂ Express ELISA Kit

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Pre-configured for analysis on MyAssays.com

Description

Limit of detection: 80% B/B₀: 36 pg/ml · Sensitivity: 50% B/B₀: 125 pg/ml · PGE₂ is one of the primary COX products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation.^1,2,3,4 Because of its involvement in inflammation, PGE₂ is the key indicator of non-steroidal anti-inflammatory drugs efficacy via inhibition of COX-1 and COX-2. Cayman Chemical’s PGE₂ Express EIA is a competitive assay that permits the rapid measurement of PGE₂ from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE₂ monoclonal, the same used in our PGE₂ Monoclonal EIA kit (Catalog No. 514010). While Catalog No. 514010 offers superior sensitivity (IC₅₀ < 50 pg/ml), the new PGE₂ Express Assay offers the convenience of a fast assay while still achieving an IC₅₀ of 125 pg/ml and a detection limit (80% B/B₀) of approximately 35 pg/ml.

1 Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E₂ in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

2 Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E₂ gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

3 Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE₂, 15-methyl PGE₂, and 16,16-dimethyl PGE₂. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

4 Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E₂ down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

Synonyms

PGE2 Express EIA Kit
Prostaglandin E2 Express ELISA Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Prostaglandin E2	100%
Prostaglandin E2 Ethanolamide	100%
Prostaglandin E2-1-glyceryl ester	100%
Prostaglandin E3	43.0%
Prostaglandin E1	18.7%
8-iso Prostaglandin E2	2.5%
Sulprostone	1.3%
6-keto Prostaglandin F1α	1.0%
8-iso Prostaglandin F2α	0.25%
Prostaglandin A2	0.04%
13,14-dihydro-15-keto Prostaglandin E2	<0.01%
Arachidonic acid	<0.01%
Arachidonoyl Ethanolamide	<0.01%
O-Arachidonoyl Ethanolamide	<0.01%
Butaprost	<0.01%
Conjugated Linoleic Acid (10E,12Z)	<0.01%
8(S),15(S)-DiHETE	<0.01%
Leukotriene B4	<0.01%
Misoprostol	<0.01%
Misoprostol (free acid)	<0.01%
tetranor-PGEM	<0.01%
tetranor-PGFM	<0.01%
Prostaglandin A1	<0.01%
8-iso Prostaglandin A2	<0.01%
Prostaglandin A3	<0.01%
Prostaglandin B1	<0.01%
Prostaglandin B2	<0.01%
Prostaglandin D2	<0.01%
11-deoxy Prostaglandin E2	<0.01%
16,16-dimethyl Prostaglandin E2	<0.01%
19(R)-hydroxy Prostaglandin E2	<0.01%
20-hydroxy Prostaglandin E2	<0.01%
15-keto Prostaglandin E2	<0.01%
Prostaglandin F1α	<0.01%
Prostaglandin F2α	<0.01%
13,14-dihydro-15-keto Prostaglandin F2α	<0.01%
Prostaglandin F3α	<0.01%
Prostaglandin J2	<0.01%
15-deoxy-Δ12,14-Prostaglandin J2	<0.01%
Thromboxane B2	<0.01%

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Background Reading

Hamberg, M., and Samuelsson, B. On the metabolism of prostaglandins E₁ and E₂ in man. J Biol Chem 246 6713-6721 (1971).

Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE₂. Prostaglandins 19 917-931 (1980).

Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE₂, 15-methyl PGE₂, and 16,16-dimethyl PGE₂. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

Antoine, C., Lellouche, J., Maclouf, J., et al. Development of enzyme immunoassays for leukotrienes using acetylcholinesterase. Biochim Biophys Acta 1075 162-168 (1991).

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE₂: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E₂ gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Wilborn, J., Crofford, L.J., Burdick, M.D., et al. Cultured lung fibroblasts isolated from patients with idiopathic pulmonary fibrosis have a diminished capacity to synthesize prostaglandin E₂ and to express cyclooxygenase-2. J Clin Invest 95 1861-1868 (1995).

Swinney, D.C., Mak, A.Y., Barnett, J., et al. Differential allosteric regulation of prostaglandin H synthase 1 and 2 by arachidonic acid. J Biol Chem 272 12393-12398 (1997).

Murakami, M., Kuwata, H., Amakasu, Y., et al. Prostaglandin E₂ amplifies cytosolic phospholipase A₂- and cyclooxygenase-2-dependent delayed prostaglandin E₂ generation in mouse osteoblastic cells. Enhancement by secretory phospholipase A₂. J Biol Chem 272 19891-19897 (1997).

Viganò, T., Habib, A., Hernandez, A., et al. Cyclooxygenase-2 and synthesis of PGE₂ in human bronchial smooth-muscle cells. Am J Respir Crit Care Med 155 864-868 (1997).

Mirzoeva, O.K., and Calder, P.C. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostaglandins Leukot Essent Fatty Acids 55 441-449 (1996).

Thibodeaux, J.K., Broussard, J.R., Godke, R.A., et al. Stimulation of progesterone production in bovine luteal cells by co-incubation with bovine blastocyst-stage embryos or trophoblastic vesicles. J Reprod Fertil 101 657-662 (1994).

Roshak, A.K., Jackson, J.R., McGough, K., et al. Manipulation of distinct NFκB proteins alters interleukin-1β-induced human rheumatoid synovial fibroblast prostaglandin E₂ formation. J Biol Chem 271 31496-31501 (1996).

Marshall, L.A., Bolognese, B., Winkler, J.D., et al. Depletion of human monocyte 85-kDa phospholipase A₂ does not alter leukotriene formation. J Biol Chem 272 759-765 (1997).

Thivierge, M., and Rola-Pleszczynski, M. Up-regulation of inducible cyclooxygenase gene expression by platelet-activating factor in activated rat alveolar macrophages. J Immunol 154 6593-6599 (1995).

Portanova, J.P., Zhang, Y., Anderson, G.D., et al. Selective neutralization of prostaglandin E₂ blocks inflammation, hyperalgesia, and interleukin 6 production in vivo. J Exp Med 184 883-891 (1996).

Ebisawa, M., Umemiya, H., Ohta, K., et al. Retinoid X receptor-antagonistic diazepinylbenzoic acids. Chem Pharm Bull 47(12) 1778-1786 (1999).

Muth-Selbach, U.S., Tegeder, I., Brune, K., et al. Acetaminophen inhibits spinal prostaglandin E₂ release after peripheral noxious stimulation. Anesthesiology 91 231-239 (1999).

Newberry, R.D., Stenson, W.F., and Lorenz, R.G. Cyclooxygenase-2-dependent arachidonic acid metabolites are essential modulators of the intestinal immune response to dietary antigen. Nat Med 5 900-906 (1999).

Oudot, F., Grynberg, A., and Sergiel, J.P. Eicosanoid synthesis in cardiomyocytes: Influence of hypoxia, reoxygenation, and polyunsaturated fatty acids. Am J Physiol 268 H308-H315 (1995).

Zhang, Y., Shaffer, A., Portanova, J., et al. Inhibition of cyclooxygenase-2 rapidly reverses inflammatory hyperalgesia and prostaglandin E₂ production. J Pharmacol Exp Ther 283 1069-1075 (1997).

Taniguchi, Y., Yokoyama, K., Inui, K., et al. Inhibition of brain cyclooxygenase-2 activity and the antipyretic action of nimesulide. Eur J Pharmacol 330 221-229 (1997).

Kuwata, H., Nakatani, Y., Murakami, M., et al. Cytosolic phospholipase A₂ is required for cytokine-induced expression of type IIA secretory phospholipase A₂ that mediates optimal cyclooxygenase-2-dependent delayed prostaglandin E₂ generation in rat 3Y1 fibroblasts. J Biol Chem 273 1733-1740 (1998).

Johnson, R.D., Polakoski, K., Everson, W.V., et al. Aspirin induces increased expression of both prostaglandin H synthase-1 and prostaglandin H synthase-2 in cultured human placental trophoblast. Am J Obstet Gynecol 177 78-85 (1997).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E₂ in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E₂ down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

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500141-96WELL

Prostaglandin E2 Express EIA Kit