Prostaglandin D2-MOX Express EIA Kit

Prostaglandin D₂-MOX Express EIA Kit

Cayman Chemical Item Number 500151

PGD₂-MOX EIA Express Kit; Prostaglandin D₂-MOX Express ELISA Kit

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Pre-configured for analysis on MyAssays.com

Description

Limit of detection: 80% B/B₀: 16 pg/ml · Sensitivity: 50% B/B₀: 75.2 pg/ml · Prostaglandin D₂ (PGD₂) is biosynthesized in the brain by a lipocalin-type PGD₂ synthase where it acts in the CNS to promote sleep induction and lowering of body temperature.¹ PGD₂ is also the major eicosanoid product of mast cells and is synthesized in large quantities by a hematopoetic-type PGD synthase during allergic and asthmatic anaphylaxis.^2,3 Measurement of the parent eicosanoid PGD₂ is appropriate in the supernatants of cell cultures, where PGD₂ levels may reach several ng/ml, and in CSF, where concentrations of several hundred pg/ml have been measured.⁴ Cayman Chemical’s PGD₂-MOX Express EIA is a competitive assay that permits the rapid measurement of PGD₂ from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in Cayman’s widely utilized PGD₂-MOX EIA (Catalog No. 512011). While Catalog No. 512011 offers superior sensitivity (IC₅₀ ~20 pg/ml), the new PGD₂-MOX Express EIA offers the convenience of a fast assay while still achieving an IC₅₀ of 75 pg/ml and a detection limit (80% B/B₀) of approximately 16 pg/ml.

1 Hayaishi, O. Sleep-wake regulation by prostaglandins D₂ and E₂. J Biol Chem 263 14593-14596 (1988).

2 Mahmud, I., Ueda, N., Yamaguchi, H., et al. Prostaglandin D synthase in human megakaryoblastic cells. J Biol Chem 272 28263-28266 (1997).

3 Matsuoka, T., Hirata, M., Tanaka, H., et al. Prostaglandin D₂ as a mediator of allergic asthma. Science 287 2013-2017 (2000).

4 Ram, A., Pandey, H.P., Matsumura, H., et al. CSF levels of prostaglandins, especially the level of prostaglandin D₂, are correlated with increasing propensity towards sleep in rats. Brain Res 751 81-89 (1997).

Synonyms

PGD2-MOX EIA Express Kit
Prostaglandin D2-MOX Express ELISA Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Prostaglandin D2-mox	100%
Prostaglandin D2	0.3%
Prostaglandin F2α	0.02%
tetranor-PGEM	<0.01%
tetranor-PGFM	<0.01%

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Background Reading

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D₂ in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

Ninomiya, H., Yu, X.Y., Hasegawa, S., et al. Endothelin-1 induces stimulation of prostaglandin synthesis in cells obtained from canine airways by bronchoalveolar lavage. Prostaglandins 43 401-411 (1992).

Spitzer, J.A. Cytokine stimulation of nitric oxide formation and differential regulation in hepatocytes and nonparenchymal cells of endotoxemic rats. Hepatology 19 217-228 (1994).

Tokugawa, Y., Kunishige, I., Kuboto, Y., et al. Lipocalin-type prostaglandin D synthase in human male reproductive organs and seminal plasma. Biol Reprod 58 600-607 (1998).

Maclouf, J., Corvazier, E., and Wang, Z. Development of a radioimmunoassay for prostaglandin D₂ using an antiserum against 11-methoxime prostaglandin D₂. Prostaglandins 31 123-132 (1986).

Ram, A., Pandey, H.P., Matsumura, H., et al. CSF levels of prostaglandins, especially the level of prostaglandin D₂, are correlated with increasing propensity towards sleep in rats. Brain Res 751 81-89 (1997).

Marshall, L.A., Bolognese, B., Winkler, J.D., et al. Depletion of human monocyte 85-kDa phospholipase A₂ does not alter leukotriene formation. J Biol Chem 272 759-765 (1997).

Hogaboam, C.M., Donigi-Gale, D., Shoupe, T.S., et al. Platelet-activating factor synthesis by peritoneal mast cells and its inhibition by two quinoline-based compounds. Br J Pharmacol 105 87-92 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D₂. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D₂ formation and characterization of its synthetases in various tissues of adult rats. Arch Biochem Biophys 260 521-531 (1988).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D₂ in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Westcott, J.Y., Murphy, R.C., and Stenmark, K. Eicosanoids in human ventricular cerebrospinal fluid following severe brain injury. Prostaglandins 34 877-887 (1987).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

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500151-96WELL

Prostaglandin D2-MOX Express EIA Kit