Luminex® Leukotriene B4 Kit

Luminex^® Leukotriene B₄ Kit

Cayman Chemical Item Number 500260

Luminex^® LTB₄ Kit

Description

Limit of detection: 80% B/B₀: 24 pg/ml · Sensitivity: 50% B/B₀: 138 pg/ml · Leukotriene B₄ (LTB₄) is a potent mediator of inflammation that stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10⁻¹⁰ M), LTB₄ causes chemotaxis and chemokinesis in human PMNLs. At higher concentrations, (1.0 x 10⁻⁷ M), LTB₄ leads to neutrophil aggregation and degranulation as well as superoxide anion production. Plasma levels of LTB₄ increase from <100 pg/ml to >100 ng/ml following leukocyte stimulation. Cayman’s Luminex^® LTB₄ Kit can be used for quantification of LTB₄ in plasma, culture media, and other sample matrices. This kit typically displays an IC₅₀ (50% B/B₀) of approximately 140 pg/ml and a detection limit (80% B/B₀) of approximately 25 pg/ml.

Synonyms

Luminex® LTB4 Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Leukotriene B4	100%
20-hydroxy Leukotriene B4	197%
20-carboxy Leukotriene B4	6.8%
6-trans Leukotriene B4	0.12
6-trans-12-epi Leukotriene B4	0.09%
5(S),6(S)-diHETE	0.02%
Arachidonic Acid	<0.01%
(±)12-HETE	<0.01%
Prostaglandin E2	<0.01%
6-keto Prostaglandin F1α	<0.01%
Thromboxane B2	<0.01%
11-dehydro Thromboxane B2	<0.01%

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Background Reading

Samuelsson, B., and Funk, C.D. Enzymes involved in the biosynthesis of leukotriene B₄. J Biol Chem 264 19469-19472 (1989).

Ford-Hutchinson, A.W. Leukotriene B₄ in inflammation. Crit Rev Immunol 10 1-12 (1990).

Mayatepek, E., and Hoffmann, G.F. Leukotrienes: Biosynthesis, metabolism, and pathophysiologic significance. Pediatr Res 37 1-9 (1995).

McMillan, R.M., and Foster, S.J. Leukotriene B₄ and inflammatory disease. Agents Actions 24 114-119 (1988).

Surette, M.E., Odeimat, A., Palmantier, R., et al. Reverse-phase high-performance liquid chromatography analysis of arachidonic acid metabolites in plasma after stimulation of whole blood ex vivo. Anal Biochem 216 392-400 (1994).

Shindo, K., Fukumura, M., and Miyakawa, K. Leukotriene B₄ levels in the arterial blood of asthmatic patients and the effects of prednisolone. Eur Respir J 8 605-610 (1995).

Doyle, M.J., Eichhold, T.H., Hynd, B.A., et al. Determination of leukotriene B₄ in human plasma by gas chromatography using a mass selective detector and a stable isotope labelled internal standard. Effect of NE-11740 on arachidonic acid metabolism. J Pharm Biomed Anal 8 137-142 (1990).

Hammarström, S., Örning, L., and Bernström, K. Metabolism of leukotrienes. Mol Cell Biochem 69 7-16 (1985).

Harper, T.W., Garrity, M.J., and Murphy, R.C. Metabolism of leukotriene B₄ in isolated rat hepatocytes. Identification of a novel 18-carboxy-19,20-dinor leukotriene B₄ metabolite. J Biol Chem 261 5414-5418 (1986).

Hansson, G., Lindgren JÅ, Dahlén, S., et al. Identification and biological activity of novel w-oxidized metabolites of leukotriene B₄ from human leukocytes. FEBS Lett 130 107-112 (1981).

Serafin, W.E., Oates, J.A., and Hubbard, W.C. Metabolism of leukotriene B₄ in the monkey. Identification of the principal nonvolatile metabolite in the urine.. Prostaglandins 27 899-911 (1984).

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500260-1ea

Luminex® Assay Buffer Concentrate (10X)

10 mL

High-Binding 96-Well Strip Plate