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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Cortisol Express EIA Kit

Cayman Chemical Item Number 500370

Cortisol Express ELISA Kit

Cortisol Express EIA Kit

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Cortisol Express EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 110 pg/ml · Sensitivity: 50% B/B0: 400 pg/ml · Cayman’s Cortisol Express EIA is a competitive assay that permits the rapid measurement of cortisol from biological samples, requiring only a two hour incubation and one hour development times. This EIA offers the convenience of a fast assay while maintaining sensitivity.
Synonyms
  • Cortisol Express ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Cortisol 100%
Prednisolone 4.0%
Cortexolone 1.6%
11-Deoxycorticosterone 0.23%
17-Hydroxyprogesterone 0.23%
Cortisol Glucuronide 0.15%
Corticosterone 0.14%
Cortisone 0.13%
Androstenedione <0.01%
Enterolactone <0.01%
Estrone <0.01%
17-Hydroxypregnenolone <0.01%
Pregnenolone <0.01%
Testosterone <0.01%
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Background Reading

Quinkler, M., and Stewart, P.M. Hypertension and the cortisol-cortisone shuttle. J Clin Endocrinol Metab 88(6) 2384-2392 (2003).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Brown, E.S., Varghese, F.P., and McEwen, B.S. Association of depression with medical illness: Does cortisol play a role? Biol Psychiatry 55 1-9 (2004).

Varghese, F.P., and Brown, E.S. The hypothalamic-pituitary-adrenal axis in major depressive disorder: A brief primer for primary care physicians. Prim Care Companion J Clin Psychiatry 3 151-155 (2001).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

Christeff, N., Michon, C., Goertz, G., et al. Abnormal free fatty acids and cortisol concentrations in the serum of AIDS patients. Eur J Cancer Clin Oncol 24(7) 1179-1183 (1988).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Aardal, E., and Holm, A. Cortisol in saliva-reference ranges and relation to cortisol in serum. Eur J Clin Chem Clin Biochem 33 927-932 (1995).

Masuzaki, H., Paterson, J., Shinyama, H., et al. A transgenic model of visceral obesity and the metabolic syndrome. Science 294 2166-2170 (2001).

Rask, E., Olsson, T., Söderberg, S., et al. Tissue-specific dysregulation of cortisol metabolism in human obesity. J Clin Endocrinol Metab 86(3) 1418-1421 (2001).

Krieger, D.T. Rhythms of ACTH and corticosteroid secretion in health and disease, and their experimental modification. J Steroid Biochem 6 785-791 (1975).

Stulnig, T.M., and Waldhäusl, W. 11β-Hydroxysteroid dehydrogenase type 1 in obesity and type 2 diabetes. Diabetologia 47 1-11 (2004).

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500370-96well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cortisol Express AChE Tracer 100 dtn
Cortisol Express EIA Monoclonal Antibody 100 dtn
Cortisol Express EIA Standard 1 ea
500370-480Solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cortisol Express AChE Tracer 500 dtn
Cortisol Express EIA Monoclonal Antibody 500 dtn
Cortisol Express EIA Standard 1 ea
500370-480well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cortisol Express AChE Tracer 500 dtn
Cortisol Express EIA Monoclonal Antibody 500 dtn
Cortisol Express EIA Standard 1 ea
500370-96Solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cortisol Express AChE Tracer 100 dtn
Cortisol Express EIA Monoclonal Antibody 100 dtn
Cortisol Express EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $283.00 $0.00
96 strip wells $283.00 $0.00
480 solid wells $1,061.00 $0.00
480 strip wells $1,061.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cortisol Express EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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UltraPure Water
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