Cysteinyl Leukotriene EIA Kit

Cayman Chemical Item Number 500390

CysLT ELISA Kit

Cysteinyl Leukotriene EIA Kit

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Cysteinyl Leukotriene EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B₀: 34 pg/ml · Sensitivity: 50% B/B₀: 103 pg/ml · Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC₄, LTD₄, and LTE₄ are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC₄ and LTD₄ are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. The biological activity of LTE₄ is much lower in most systems studied, but its presence reflects the prior existence of LTC₄ and LTD₄. Cayman’s CysLT EIA Kit is a competitive assay based on a proprietary high-affinity monoclonal antibody that can be used for quantification of CysLT in urine and other sample matrices. The EIA typically displays an IC₅₀ (50% B/B₀) of approximately 103 pg/ml and a detection limit (80% B/B₀) of approximately 34 pg/ml.

Synonyms

CysLT ELISA Kit

Stability

6 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Specificity

Leukotriene C4	100%
Leukotriene D4	100%
Leukotriene E4	79%
5,6-DiHETE	3.7%
Leukotriene B4	1.3%
5(S)-HETE	0.04%
Arachidonic Acid	<0.01%

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Background Reading

Cai, C., Yang, J., Hu, S., et al. Relationship between urinary cysteinyl leukotriene E₄ levels and clinical response to antileukotriene treatment in patients with asthma. Lung 185 105-112 (2007).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

Piper, P.J. Formation and actions of leukotrienes. Physiol Rev 64 744-761 (1984).

Liu, J., García-Cardeña, G., and Sessa, W.C. Palmitoylation of endothelial nitric oxide synthase is necessary for optimal stimulated release of nitric oxide. Implications for caveolae localization. Biochemistry 35 13277-13281 (1996).

Matsumoto, S., Hayashi, Y., Kinoshita, I., et al. Immunoaffinity purification of prostaglandin E₂ and leukotriene C₄ prior to radioimmunoassay: Application to human synovial fluid. Ann Clin Biochem 30(Part I) 60-68 (1993).

Campbell, B.J., Baker, S.F., Shukla, S.D., et al. Bioconversion of leukotriene D₄ by lung dipeptidase. Biochim Biophys Acta 1042 107-112 (1990).

Maclouf, J., Corvazier, E., and Wang, Z. Development of a radioimmunoassay for prostaglandin D₂ using an antiserum against 11-methoxime prostaglandin D₂. Prostaglandins 31 123-132 (1986).

Miyata, A., Hara, S., Yokoyama, C., et al. Molecular cloning and expression of human prostacyclin synthase. Biochem Biophys Res Commun 200 1728-1734 (1994).

Mita, H., Turikisawa, N., Yamada, T., et al. Quantification of leukotriene B₄ glucuronide in human urine. Prostaglandins Other Lipid Mediat 83 42-49 (2007).

Hardy, G., Boizel, R., Bessard, J., et al. Urinary leukotriene E₄ excretion is increased in type 1 diabetic patients a quantification by liquid chromatography-tandem mass spectrometry. Prostaglandins Other Lipid Mediat 78 291-299 (2005).

Dahlén, S., Björk, J., Hedqvist, P., et al. Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: In vivo effects with relevance to the acute inflammatory response. Proc Natl Acad Sci USA 78 3887-3891 (1981).

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500390-96 solid

Cysteinyl Leukotriene EIA Kit

Cysteinyl Leukotriene EIA Kit

Cysteinyl Leukotriene EIA Kit Analysis Tools

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