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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

STAT-8-Isoprostane EIA Kit

Cayman Chemical Item Number 500431

STAT-8-Isoprostane ELISA Kit; iPF2α-III Alkaline Phosphatase EIA Kit; STAT-8-iso Prostaglandin F2α EIA Kit; STAT-8-epi Prostaglandin F2α EIA Kit

STAT-8-Isoprostane EIA Kit

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STAT-8-Isoprostane EIA Kit Analysis Tools

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Description

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. At least one of the isoprostanes, 8-isoprostane (8-iso PGF2α), has biological activity and has implicated as a causative mediator and specific marker of free radical-induced damage in a variety of diseases related to oxidative stress.1,2,3,4 Cayman Chemical’s STAT-8-isoprostane EIA is a competitive assay that permits the rapid measurement of 8-iso PGF2α from biological samples, requiring only 1 hour incubation and development times for each step. This assay format is the similar to that employed in Cayman’s popular 8-isoprostane EIA (Catalog No. 5126351) with the only change being the use of an alkaline phosphatase tracer in place of an AChE tracer. While Catalog No. 516351 offers superior sensitivity (IC50 ~35 pg/ml), the new STAT-8-isoprostane Assay offers the convenience of a fast assay while still achieving an IC50 of 180 pg/ml and a detection limit (80% B/B0) of approximately 45 pg/ml.

1 Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF2α, in rabbit lung in situ. Am J Physiol 263 H660-H663 (1992).

2 Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF2α, in pulmonary oxygen toxicity. J Appl Physiol 77 2912-2917 (1994).

3 Praticò, D., Lawson, J.A., Rokach, J., et al. The isoprostanes in biology and medicine. Trends Endocrinol Metab 12(6) 243-247 (2001).

4 Greco, A., Minghetti, L., and Levi, G. Isoprostanes, novel markers of oxidative injury, help understanding the pathogenesis of neurodegenerative diseases. Neurochem Res 25(9/10) 1357-1364 (2000).

Synonyms
  • STAT-8-Isoprostane ELISA Kit
  • iPF2α-III Alkaline Phosphatase EIA Kit
  • STAT-8-iso Prostaglandin F2α EIA Kit
  • STAT-8-epi Prostaglandin F2α EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
8-iso Prostaglandin F2α 100%
8-iso Prostaglandin F3α 20.55%
2,3-dinor-8-iso Prostaglandin F2α 4.00%
8-iso Prostaglandin E2 1.84%
2,3-dinor-8-iso Prostaglandin F1α 1.7%
8-iso Prostaglandin E1 1.56%
Prostaglandin F1α 0.71%
Prostaglandin F3α 0.66%
Prostaglandin E1 0.39%
Prostaglandin D2 0.16%
6-keto Prostaglandin F1α 0.14%
Prostaglandin F2α 0.14%
2,3-dinor-6-keto Prostaglandin F1α 0.09%
8-iso Prostaglandin F1β 0.08%
Thromboxane B2 0.08%
11-dehydro Thromboxane B2 0.07%
11β-Prostaglandin F2α 0.03%
Prostaglandin E2 0.02%
8-iso-15(R)-Prostaglandin F2α 0.02%
13,14-dihydro-15-keto Prostaglandin F2α <0.01%
tetranor-PGEM <0.01%
tetranor-PGFM <0.01%
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500431-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Tris Buffer Concentrate (10X) 4 × 10 mL
DEA Buffer Concentrate (10X) 12.5 mL
pNPP Tablets 25 ea
STAT-8-Isoprostane AP Tracer 500 dtn
STAT-8-Isoprostane EIA Antiserum 500 dtn
STAT-8-Isoprostane EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 12.5 mL
500431-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Tris Buffer Concentrate (10X) 4 × 10 mL
DEA Buffer Concentrate (10X) 12.5 mL
pNPP Tablets 25 ea
STAT-8-Isoprostane AP Tracer 500 dtn
STAT-8-Isoprostane EIA Antiserum 500 dtn
STAT-8-Isoprostane EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 12.5 mL
5000431-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Tris Buffer Concentrate (10X) 2 × 10 mL
DEA Buffer Concentrate (10X) 2.5 mL
pNPP Tablets 5 ea
STAT-8-Isoprostane AP Tracer 100 dtn
STAT-8-Isoprostane EIA Antiserum 100 dtn
STAT-8-Isoprostane EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 5 mL
500431-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Tris Buffer Concentrate (10X) 2 × 10 mL
DEA Buffer Concentrate (10X) 2.5 mL
pNPP Tablets 5 ea
STAT-8-Isoprostane AP Tracer 100 dtn
STAT-8-Isoprostane EIA Antiserum 100 dtn
STAT-8-Isoprostane EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 5 mL
Size Price Quantity Subtotal
96 solid wells $250.00 $0.00
96 strip wells $250.00 $0.00
480 solid wells $938.00 $0.00
480 strip wells $938.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample STAT-8-Isoprostane EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

8-iso Prostaglandin F2α
8-Isoprostane EIA Kit
Sampling Tubes with BHT
SPE Cartridges (C-18)
UltraPure Water
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