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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Prostaglandin D2 FPIA Kit - Green

Cayman Chemical Item Number 500581

PGD2 Fluorescence Polarization Immunoassay - Green

Prostaglandin D2 FPIA Kit - Green

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Description

Limit of detection: 410 pg/ml · Prostaglandin D2 (PGD2) is biosynthesized in the brain by a glutathione-independent lipocalin-type PGD2 synthase (L-PGDS).1 It accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans.2 PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase (hematopoietic PGD Synthase; H-PGDS).3 In this setting, PGD2 and acts as a pro-inflammatory mediator in allergic reactions following release in large amounts from allergen-stimulated mast cells. Cayman’s PGD2 Fluorescence Polarization Immunoassay (FPIA) is especially designed for High Throughput Screening (HTS) applications for direct, rapid measurement of PGD2. The assay is particularly suited for samples from cell culture and purified enzyme preparations. The PGD2-FPIA is robust (Z' = 0.58), exhibits greater than 150 mP over a range of 164 pg/ml to 100 ng/ml PGD2, and has a detection limit of 410 pg/ml.

1 Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

2 Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

3 Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Synonyms
  • PGD2 Fluorescence Polarization Immunoassay - Green
Stability 1 year
Storage -80°C
Shipping Dry Ice in continental US; may vary elsewhere
Specificity
Prostaglandin D2 100%
Prostaglandin F2α 100%
Thromboxane B2 4.98%
12-HHTrE 0.71%
Prostaglandin E2 0.25%
6-keto Prostaglandin F1α 0.10%
Arachidonic Acid 0.04%
tetranor-PGEM 0.01%
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Background Reading

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D2 formation and characterization of its synthetases in various tissues of adult rats. Arch Biochem Biophys 260 521-531 (1988).

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500581-384WELL
384-Well Solid Plate (black; non-binding)
Prostaglandin D2 FPIA Reagent - Green 400 dtn
Prostaglandin D2 FPIA Standard - Green 1 ea
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
500581-1920WELL
384-Well Solid Plate (black; non-binding)
Prostaglandin D2 FPIA Reagent - Green 2000 dtn
Prostaglandin D2 FPIA Standard - Green 1 ea
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Size Price Quantity Subtotal
384 wells $305.00 $0.00
1920 wells $1,144.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin D2 FPIA Kit - Green under the Cayman Challenge program.

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Let Cayman analyze your samples for you. See EIA Service for details and availability.

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