Limit of detection:
80% B/B0: 30 pg/ml
·
Sensitivity:
50% B/B0: 150 pg/ml
·
Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.1 Biologists often measure corticosterone in fecal material of the species they are studying.1,2 This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.1 Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.2,3 The concentration of corticosterone in human plasma is approximately 0.9-3.9 ng/ml in females and 3.2-7.6 ng/ml in males.4 Cayman’s Corticosterone EIA Kit is a competitive assay that can be used for quantification of corticosterone in plasma and cell culture supernatant samples.
1
Goymann, W., Möstl, E., and Gwinner, E. Corticosterone metabolites can be measured noninvasively in excreta of European stonechats (Saxicola torquata rubicola). Auk 119(4) 1167-1173 (2002).
2
Vázquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., et al. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav 70 305-310 (2001).
3
Millspaugh, J.J., Washburn, B.E., Milanick, M.A., et al. Effects of heat and chemical treatments on fecal glucocorticoid measurements: Implications for sample transport. Wildl Soc Bull 31(2) 399-406 (2003).
4
Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).
Vázquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., et al. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav 70 305-310 (2001).
Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).
Goymann, W., Möstl, E., and Gwinner, E. Corticosterone metabolites can be measured noninvasively in excreta of European stonechats (Saxicola torquata rubicola). Auk 119(4) 1167-1173 (2002).
Millspaugh, J.J., Washburn, B.E., Milanick, M.A., et al. Effects of heat and chemical treatments on fecal glucocorticoid measurements: Implications for sample transport. Wildl Soc Bull 31(2) 399-406 (2003).
Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).
Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).
Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).
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