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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Corticosterone EIA Kit

Cayman Chemical Item Number 500655

Corticosterone EIA Kit

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Corticosterone EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 30 pg/ml · Sensitivity: 50% B/B0: 150 pg/ml · Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.1 Biologists often measure corticosterone in fecal material of the species they are studying.1,2 This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.1 Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.2,3 The concentration of corticosterone in human plasma is approximately 0.9-3.9 ng/ml in females and 3.2-7.6 ng/ml in males.4 Cayman’s Corticosterone EIA Kit is a competitive assay that can be used for quantification of corticosterone in plasma and cell culture supernatant samples.

1 Goymann, W., Möstl, E., and Gwinner, E. Corticosterone metabolites can be measured noninvasively in excreta of European stonechats (Saxicola torquata rubicola). Auk 119(4) 1167-1173 (2002).

2 Vázquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., et al. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav 70 305-310 (2001).

3 Millspaugh, J.J., Washburn, B.E., Milanick, M.A., et al. Effects of heat and chemical treatments on fecal glucocorticoid measurements: Implications for sample transport. Wildl Soc Bull 31(2) 399-406 (2003).

4 Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Corticosterone 100%
11-Dehydrocorticosterone 11%
11-Deoxycorticosterone 7%
Progesterone 0.31%
Cortisol 0.17%
Aldosterone 0.06%
Testosterone 0.03%
Pregnenolone 0.02%
5α-DHT 0.01%
Androstenedione <0.01%
Cortisone <0.01%
DHEA <0.01%
DHEA-S <0.01%
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Background Reading

Vázquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., et al. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav 70 305-310 (2001).

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

Goymann, W., Möstl, E., and Gwinner, E. Corticosterone metabolites can be measured noninvasively in excreta of European stonechats (Saxicola torquata rubicola). Auk 119(4) 1167-1173 (2002).

Millspaugh, J.J., Washburn, B.E., Milanick, M.A., et al. Effects of heat and chemical treatments on fecal glucocorticoid measurements: Implications for sample transport. Wildl Soc Bull 31(2) 399-406 (2003).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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500655-96SOLID
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Corticosterone AChE Tracer 100 dtn
Corticosterone EIA Antiserum 100 dtn
Corticosterone EIA Standard 1 ea
Rabbit Anti-Sheep IgG Coated Solid Plate 1 ea
500655-480SOLID
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Corticosterone AChE Tracer 500 dtn
Corticosterone EIA Antiserum 500 dtn
Corticosterone EIA Standard 1 ea
Rabbit Anti-Sheep IgG Coated Solid Plate 5 ea
500655-480well
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Corticosterone AChE Tracer 500 dtn
Corticosterone EIA Antiserum 500 dtn
Corticosterone EIA Standard 1 ea
Rabbit Anti-Sheep IgG Precoated Strip Plate 5 ea
500655-96well
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Corticosterone AChE Tracer 100 dtn
Corticosterone EIA Antiserum 100 dtn
Corticosterone EIA Standard 1 ea
Rabbit Anti-Sheep IgG Precoated Strip Plate 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Corticosterone EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Testosterone EIA Kit
UltraPure Water
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