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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

PAD4 Autoantibody EIA Kit

Cayman Chemical Item Number 500930

Protein Arginine Deiminase 4; Peptidylartginine Deaminase 4

PAD4 Autoantibody EIA Kit

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Description

Protein arginine deiminase 4 (peptidylarginine deiminase 4 (PAD4)), is a guanidino-modifying enzyme which catalyzes the conversion of specific arginine residues to citrulline in a calcium-dependent manner. PAD4 activity is increased in rheumatoid arthritis (RA), producing citrulline-containing proteins (CCP) that induce the formation of arthritogenic autoantibodies. In addition to anti-CCP autoantibodies, 23-45% of RA patients also produce autoantibodies specific for the PAD4 enzyme itself. Cayman’s PAD4 Autoantibody EIA Kit is an immunometric assay which can be used to measure anti-PAD4 autoantibodies of any isotype (IgM, IgG, and IgA) in human plasma and serum without prior sample purification. Affinity-purified anti-PAD4 antibody isolated from the plasma of a patient with RA is used as the standard. One unit is approximately equal to 1 ng of anti-PAD4 Ig protein. The standard curve spans the range of 0-2,000 U/ml, with an LLOQ of 62.5 U/ml.
Synonyms
  • Protein Arginine Deiminase 4
  • Peptidylartginine Deaminase 4
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Shirai, H., Blundell, T.L., and Mizuguchi, K. A novel superfamily of enzymes that catalyze the modification of guanidino groups. Trends Biochem Sci 26(8) 465-468 (2001).

Takizawa, Y., Sawada, T., Suzuki, A., et al. Peptidylarginine deiminase 4 (PADI4) identified as a conformation-dependent autoantigen in rheumatoid arthritis. Scand J Rheumatol 34(3) 212-5 (2005).

Zhao, J., Zhao, Y., He, J., et al. Prevalence and significance of anti-peptidylarginine deiminase 4 antibodies in rheumatoid arthritis. J Rheumatol 35(6) 969-974 (2008).

Halvorsen, E.H., Pollmann, S., Gilboe, I., et al. Serum IgG antibodies to peptidylarginine deiminase 4 in rheumatoid arthritis and associations with disease severity. Ann Rheum Dis 67 414-417 (2008).

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500930-96well
HRP Stop Solution 12 mL
96-Well Cover Sheet
Polysorbate 20 1 ea
Orange Non-EIA Kit Box (96 Well)
Non-fat Dry Milk Assay Reagent 2 × 5 g
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
TMB Substrate Solution 12 mL
Goat Anti-Human Ig (H+L)/HRP Conjugate 2 × 1 ea
PAD4 Precoated 96-Well Strip Plate 1 ea
Anti-PAD4 (human) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $425.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample PAD4 Autoantibody EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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