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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Prostaglandin D2 Express EIA Kit

Cayman Chemical Item Number 512041

PGD2 Express ELISA Kit

Prostaglandin D2 Express EIA Kit

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Prostaglandin D2 Express EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 350 pg/ml · Sensitivity: 50% B/B0: 1,300 pg/ml · Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF2α and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman’s PGD2 Express EIA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions. This EIA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an IC50 value (50% B/B0) of approximately 1,300 pg/ml and a detection limit (80% B/B0) of approximately 350 pg/ml.
Synonyms
  • PGD2 Express ELISA Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Specificity
Prostaglandin D2 100%
Prostaglandin F2α 92.4%
Prostaglandin J2 21.6%
Prostaglandin E2 2.86%
Thromboxane B2 2.54%
11β-Prostaglandin F2α 1.99%
8-iso Prostaglandin F2α 1.90%
Prostaglandin A2 0.72%
12(S)-HHTrE 0.16%
6-keto Prostaglandin F1α 0.05%
13,14-dihydro-15-keto Prostaglandin D2 0.02%
Arachidonic Acid <0.01%
Leukotriene D4 <0.01%
tetranor-PGDM <0.01%
tetranor-PGEM <0.01%
tetranor-PGFM <0.01%
tetranor-PGJM <0.01%
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Background Reading

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Ram, A., Pandey, H.P., Matsumura, H., et al. CSF levels of prostaglandins, especially the level of prostaglandin D2, are correlated with increasing propensity towards sleep in rats. Brain Res 751 81-89 (1997).

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512041-480WELL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Green EIA Kit Box (480 Well)
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin D2 Express AChE Tracer 500 dtn
Prostaglandin D2 Express EIA Monoclonal Antibody 500 dtn
Prostaglandin D2 Express EIA Standard 1 ea
512041-96Solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Blue EIA Kit Box (96 Well)
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin D2 Express AChE Tracer 100 dtn
Prostaglandin D2 Express EIA Monoclonal Antibody 100 dtn
Prostaglandin D2 Express EIA Standard 1 ea
512041-480Solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Green EIA Kit Box (480 Well)
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin D2 Express AChE Tracer 500 dtn
Prostaglandin D2 Express EIA Monoclonal Antibody 500 dtn
Prostaglandin D2 Express EIA Standard 1 ea
512041-96WELL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Blue EIA Kit Box (96 Well)
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin D2 Express AChE Tracer 100 dtn
Prostaglandin D2 Express EIA Monoclonal Antibody 100 dtn
Prostaglandin D2 Express EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $342.00 $0.00
96 strip wells $342.00 $0.00
480 solid wells $1,283.00 $0.00
480 strip wells $1,283.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin D2 Express EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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