See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Prostaglandin D2 FPIA Kit - Red

Cayman Chemical Item Number 512051

PGD2 Fluorescence Polarization Immunoassay - Red

Prostaglandin D2 FPIA Kit - Red

See more

Description

Limit of detection: 550 pg/ml · This FPIA is offered with a 96-well plate or a 384-well plate, the other components are all the same between the two sizes. Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kD glutathione-independent lipocalin-type PGD2 synthase (L-PGDS). PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase (hematopoietic PGD Synthase; H-PGDS). Cayman’s PGD2 Fluorescence Polarization Immunoassay (FPIA) is especially designed for the rapid measurement of PGD2 from cell culture and purified enzyme preparation. The PGD2-FPIA is robust (Z’ = 0.62), exhibits greater than 180 mP over a range of 340 pg/ml to 100 ng/ml PGD2, and has a detection limit of 550 pg/ml.
Synonyms
  • PGD2 Fluorescence Polarization Immunoassay - Red
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Specificity
Prostaglandin F2α 118.46%
Prostaglandin D2 100%
Prostaglandin J2 24.40%
11β-Prostaglandin F2α 4.13%
12(S)-HHTrE 3.58%
Thromboxane B2 3.47%
Prostaglandin E2 2.54%
6-keto Prostaglandin F1α 0.46%
13,14-dihydro-15-keto Prostaglandin D2 0.08%
Arachidonic Acid 0.06%
Show all 10 Hide all but first 3

Background Reading

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Song, W., Wang, M., Ricciotti, E., et al. Tetranor PGDM, an abundant urinary metabolite reflects biosynthesis of prostaglandin D2 in mice and humans. J Biol Chem 283(2) 1179-1188 (2008).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D2 formation and characterization of its synthetases in various tissues of adult rats. Arch Biochem Biophys 260 521-531 (1988).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

Show all 8 Hide all but first 3
512051-384well
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 400 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-96well
96-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 400 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-1920well
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 2000 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-480well
96-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 2000 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
Size Price Quantity Subtotal
384 wells $305.00 $0.00
96 wells $305.00 $0.00
1920 wells $1,144.00 $0.00
480 wells $1,144.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin D2 FPIA Kit - Red under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Prostaglandin D2
Prostaglandin D2 EIA Kit
Prostaglandin D2 Express EIA Kit
Prostaglandin D2 FPIA Kit - Green
Prostaglandin D2-MOX EIA Kit
Prostaglandin D2-MOX Express EIA Kit
Prostaglandin D Synthase Inhibitor Screening Assay Kit
Prostaglandin E2 FPIA Kit - Green
Prostaglandin E2 FPIA Kit - Red
UltraPure Water
Show all 10 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?