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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Prostaglandin Screening EIA Kit

Cayman Chemical Item Number 514012

Prostaglandin Screening ELISA Kit; PG Screening EIA Kit

Prostaglandin Screening EIA Kit

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Description

Limit of detection: 80% B/B0: 29 pg/ml · Sensitivity: 50% B/B0: 222 pg/ml · This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE2 assay (Item No. 514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples tedious. The specificity of the PGE2 assay also eliminates quantification of other PGs which may be produced by the cells. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay. The broad standard curve minimizes the need for sample dilution. Because this assay will also recognize thromboxane B2 (TXB2), cell culture media must not contain fetal calf serum (FCS). The high concentration of TXB2 in serum (approximately 400 ng/ml) will completely displace the tracer, making PG measurement impossible.
Synonyms
  • Prostaglandin Screening ELISA Kit
  • PG Screening EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Prostaglandin E1 100%
Prostaglandin E2 100%
Prostaglandin F1α 100%
Prostaglandin F2α 100%
Prostaglandin F3α 51.3%
Prostaglandin E2 Ethanolamide 44.0%
6-keto Prostaglandin F1α 43.6%
8-iso Prostaglandin F2α 38.4%
8-iso Prostaglandin E2 28.5%
Prostaglandin D2 26.6%
8-iso-2,3-dinor Prostaglandin F1α 20.0%
Prostaglandin E3 9.5%
Thromboxane B2 5.0%
12(S)-HHTrE 0.25%
Leukotriene D4 0.2%
Arachidonic Acid <0.01%
Leukotriene B4 <0.01%
Leukotriene C4 <0.01%
Leukotriene E4 <0.01%
Misoprostol <0.01%
Misoprostol (free acid) <0.01%
Prostaglandin A1 <0.01%
Prostaglandin A2 <0.01%
Prostaglandin A3 <0.01%
Prostaglandin B1 <0.01%
15-keto Prostaglandin E2 <0.01%
13,14-dihydro-15-keto Prostaglandin F2α <0.01%
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Background Reading

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

514012-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin Screening AChE Tracer 500 dtn
Prostaglandin Screening EIA Antiserum 500 dtn
Prostaglandin Screening EIA Standard 1 ea
514012-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin Screening AChE Tracer 500 dtn
Prostaglandin Screening EIA Antiserum 500 dtn
Prostaglandin Screening EIA Standard 1 ea
514012-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin Screening AChE Tracer 100 dtn
Prostaglandin Screening EIA Antiserum 100 dtn
Prostaglandin Screening EIA Standard 1 ea
514012-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin Screening AChE Tracer 100 dtn
Prostaglandin Screening EIA Antiserum 100 dtn
Prostaglandin Screening EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin Screening EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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UltraPure Water
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