Limit of detection: 80% B/B₀: 2 pg/ml · Sensitivity: 50% B/B₀: 11 pg/ml · Prostaglandin E₂ (PGE₂) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE₂. Thus, cultured endothelial cells or osteoblasts will release PGE₂ into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE₂ from the medium is a good way to measure PGE₂ production from these cells. PGE₂ is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE₂ cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE₂, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE₂ production. Cayman’s PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA₂ and 13,14-dihydro-15-keto PGE₂ to a single, stable derivative that could be easily quantified by EIA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection.

Bothwell, W., Verburg, M., Wynalda, M., et al. A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E₁ and E₂: An indirect index of their in vivo disposition and pharmacokinetics. J Pharmacol Exp Ther 220 229-235 (1982).

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE₂: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Hinson, R.M., Williams, J.A., and Shacter, E. Elevated interleukin 6 is induced by prostaglandin E₂ in a murine model of inflammation: Possible role of cyclooxygenase-2. Proc Natl Acad Sci USA 93 4885-4890 (1996).

Hong, J., and Lee, L. Cigarette smoke-induced bronchoconstriction: Causative agents and role of thromboxane receptors. J Appl Physiol 78 2260-2266 (1995).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Stein, T.P., Schluter, M.D., and Moldawer, L.L. Endocrine relationships during human spaceflight. Am J Physiol Endocrinol Metab 276 E155-E162 (1999).

Muscará, M.N., McKnight, W., Asfaha, S., et al. Wound collagen deposition in rats: Effects of an NO-NSAID and a selective COX-2 inhibitor. Br J Pharmacol 129 681-686 (2000).

Show all 9 Hide all but first 3