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iPF2α-VI EIA Kit

Cayman Chemical Item Number 516301

iPF2α-VI ELISA Kit

iPF2α-VI EIA Kit

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iPF2α-VI EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 50 pg/ml · Sensitivity: 50% B/B0: 250 pg/ml · The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by the ingestion of alcohol, carbon tetrachloride and cigarette smoke. There are four main families or classes of isoprostanes (Classes III, IV, V, and VI).1,2,3 It is ironic that the best studied class, the iPF2α-III series, is also the least abundant. This assay is the first simple, reliable EIA method for the measurement of the more prominent iPF2α-VI isoprostane. Normal urinary levels of iPF2α-VI are 500-700 pg/mg creatinine, whereas the III series compound 8-isoprostane (iPF2α-III) is present at about 150 pg/mg creatinine. Cayman Chemical’s iPF2α-VI EIA is a competitive assay that can be used for the quantification of iPF2α-VI from plasma, urine, cultured cells, and tissues.

1 Li, H., Lawson, J.A., Reilly, M., et al. Quantitative high performance liquid chromatography/tandem mass spectrometric analysis of the four classes of F2-isoprostanes in human urine. Proc Natl Acad Sci USA 96(23) 13381-13386 (1999).

2 Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87 9383-9387 (1990).

3 Waugh, R.J., and Murphy, R.C. Mass spectrometric anaysis of four regioisomers of F2-isoprostanes formed by free radical oxidation of arachidonic acid. J Am Soc Mass Spectrom 7 490-499 (1996).

Synonyms
  • iPF2α-VI ELISA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
iPF2α-VI 100%
8,12-epi iPF2α-VI 0.5%
Prostaglandin D2 0.01%
8,12-epi iPF2α-III <0.01%
Prostaglandin E1 <0.01%
8-iso Prostaglandin E1 <0.01%
Prostaglandin E2 <0.01%
8-iso Prostaglandin E2 <0.01%
8-iso-15-keto-Prostaglandin E2 <0.01%
Prostaglandin F1α <0.01%
2,3-dinor-8-iso Prostaglandin F1α <0.01%
8-iso Prostaglandin F1α <0.01%
6-keto Prostaglandin F1α <0.01%
8-iso Prostaglandin F1β <0.01%
Prostaglandin F2α <0.01%
11β-Prostaglandin F2α <0.01%
8-iso Prostaglandin F2α <0.01%
8-iso-15(R) Prostaglandin F2α <0.01%
8-iso-15-keto Prostaglandin F2α <0.01%
8-iso-13,14-dihydro-15-keto Prostaglandin F2α <0.01%
13,14-dihydro-15-keto Prostaglandin F2α <0.01%
2,3-dinor-8-iso Prostaglandin F2α <0.01%
8-iso Prostaglandin F2β <0.01%
Prostaglandin F3α <0.01%
Thromoboxane B2 <0.01%
11-dehydro Thromboxane B2 <0.01%
tetranor-PGEM <0.01%
tetranor-PGFM <0.01%
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Background Reading

Li, H., Lawson, J.A., Reilly, M., et al. Quantitative high performance liquid chromatography/tandem mass spectrometric analysis of the four classes of F2-isoprostanes in human urine. Proc Natl Acad Sci USA 96(23) 13381-13386 (1999).

Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87 9383-9387 (1990).

Praticò, D., Barry, O.P., Lawson, J.A., et al. IPF2α-I: An index of lipid peroxidation in humans. Proc Natl Acad Sci USA 95 3449-3454 (1998).

Morrow, J.D., Harris, T.M., and Roberts, L.J. Noncyclooxygenase oxidative formation of a series of novel prostaglandins: Analytical ramifications for measurement of eicosanoids. Anal Biochem 184 1-10 (1990).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Waugh, R.J., and Murphy, R.C. Mass spectrometric anaysis of four regioisomers of F2-isoprostanes formed by free radical oxidation of arachidonic acid. J Am Soc Mass Spectrom 7 490-499 (1996).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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516301-480solid
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
iPF2α-VI-AChE Tracer 500 dtn
iPF2α-VI EIA Antiserum 500 dtn
iPF2α-VI EIA Standard 1 ea
516301-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
iPF2α-VI-AChE Tracer 100 dtn
iPF2α-VI EIA Antiserum 100 dtn
iPF2α-VI EIA Standard 1 ea
516301-96solid
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
iPF2α-VI-AChE Tracer 100 dtn
iPF2α-VI EIA Antiserum 100 dtn
iPF2α-VI EIA Standard 1 ea
516301-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
iPF2α-VI-AChE Tracer 500 dtn
iPF2α-VI EIA Antiserum 500 dtn
iPF2α-VI EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $246.00 $0.00
96 strip wells $246.00 $0.00
480 solid wells $923.00 $0.00
480 strip wells $923.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample iPF2α-VI EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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