8-Isoprostane EIA Kit

Cayman Chemical Item Number 516351

iPF_2α-III EIA Kit; 8-epi Prostaglandin F_2α EIA Kit; 8-iso Prostaglandin F_2α EIA Kit; 8-Isoprostane ELISA Kit

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Pre-configured for analysis on MyAssays.com

Description

Limit of detection: 80% B/B₀: 2.7 pg/ml · Sensitivity: 50% B/B₀: 10 pg/ml · The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF_2α), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor¹ and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.² 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.³ 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.⁴ Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.⁵ Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.^5,6

1 Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF_2α, in rabbit lung in situ. Am J Physiol 263 H660-H663 (1992).

2 Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF_2α, in pulmonary oxygen toxicity. J Appl Physiol 77 2912-2917 (1994).

3 Morrow, J.D., Frei, B., Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. N Engl J Med 332 1198-1203 (1995).

4 Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87 9383-9387 (1990).

5 Wang, Z., Ciabattoni, G., Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F_2α excretion in man. J Pharmacol Exp Ther 275 94-100 (1995).

6 Reilly, M.P., Barry, P., Lawson, J.A., et al. Urinary 8-epi PGF_2α: An index of oxidant stress in vivo. Fibrinolysis & Proteolysis 11 81-84 (1997).

Synonyms

iPF2α-III EIA Kit
8-epi Prostaglandin F2α EIA Kit
8-iso Prostaglandin F2α EIA Kit
8-Isoprostane ELISA Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

8-Isoprostane	100%
8-iso Prostaglandin F2α ethanolamide	100%
8-iso Prostaglandin F3α	20.6%
2,3-dinor-8-iso Prostaglandin F2α	4.00%
8-iso Prostaglandin E2	1.84%
2,3-dinor-8-iso Prostaglandin F1α	1.7%
8-iso Prostaglandin E1	1.56%
Prostaglandin F1α	0.71%
Prostaglandin F3α	0.66%
Prostaglandin E1	0.39%
Prostaglandin D2	0.16%
6-keto Prostaglandin F1α	0.14%
Prostaglandin F2α	0.14%
2,3-dinor-6-keto Prostaglandin F1α	0.09%
8-iso Prostaglandin F1β	0.08%
Thromboxane B2	0.08%
11-dehydro Thromboxane B2	0.07%
11β-Prostaglandin F2α	0.03%
Prostaglandin E2	0.02%
8-iso-15(R)-Prostaglandin F2α	0.02%
8,12-epi iPF2α-III	<0.01%
iPF2α-VI	<0.01%
8,12-epi iPF2α-VI	<0.01%
tetranor-PGEM	<0.01%
tetranor-PGFM	<0.01%
13,14-dihydro-15-keto Prostaglandin F2α	<0.01%

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Background Reading

Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87 9383-9387 (1990).

Morrow, J.D., Harris, T.M., and Roberts, L.J. Noncyclooxygenase oxidative formation of a series of novel prostaglandins: Analytical ramifications for measurement of eicosanoids. Anal Biochem 184 1-10 (1990).

Bligh, E.G., and Dyer, W.J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37 911-917 (1959).

Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF_2α, in rabbit lung in situ. Am J Physiol 263 H660-H663 (1992).

Morrow, J.D., Awad, J.A., Boss, H.J., et al. Non-cyclooxygenase-derived prostanoids (F2-isoprostanes) are formed in situ on phospholipids. Proc Natl Acad Sci USA 89 10721-10725 (1992).

Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF_2α, in pulmonary oxygen toxicity. J Appl Physiol 77 2912-2917 (1994).

Nanji, A.A., Khwaja, S., Tahan, S.R., et al. Plasma levels of a novel noncyclooxygenase-derived prostanoid (8-isoprostane) correlate with severity of liver injury in experimental alcoholic liver disease. J Pharmacol Exp Ther 269 1280-1285 (1994).

Wang, Z., Ciabattoni, G., Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F_2α excretion in man. J Pharmacol Exp Ther 275 94-100 (1995).

Morrow, J.D., Frei, B., Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. N Engl J Med 332 1198-1203 (1995).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Natarajan, R., Lanting, L., Gonzales, N., et al. Formation of an F2-isoprostane in vascular smooth muscle cells by elevated glucose and growth factors. Am J Physiol 271 H159-H165 (1996).

Hoffman, S.W., Roof, R.L., and Stein, D.G. A reliable and sensitive enzyme immunoassay method for measuring 8-iso prostaglandin F_2α: A marker for lipid peroxidation after experimental brain injury. J Neurosci Methods 68 133-136 (1996).

Reilly, M.P., Barry, P., Lawson, J.A., et al. Urinary 8-epi PGF_2α: An index of oxidant stress in vivo. Fibrinolysis & Proteolysis 11 81-84 (1997).

Hart, C.M., Karman, R.J., Blackburn, T.L., et al. Role of 8-epi PGF_2α, 8-isoprostane, in H₂O₂-induced derangements of pulmonary artery endothelial cell barrier function. Prostaglandins Leukot Essent Fatty Acids 58 9-16 (1998).

Vacchiano, C.A., Osborne, G.R., and Tempel, G.E. 8-iso-PGF_2α production by alveolar macrophages exposed to hyperoxia. Shock 9 266-273 (1998).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Cusson, M., Laforge, M., Miller, D., et al. Functional significance of parasitism-induced suppression of juvenile hormone esterase activity in developmentally delayed choristoneura fumiferana larvae. Gen Comp Endocrinol 117 343-354 (2000).

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516351-480WELL

8-Isoprostane EIA Kit