iPF_2α-III EIA Kit; 8-epi Prostaglandin F_2α EIA Kit; 8-iso Prostaglandin F_2α EIA Kit; 8-Isoprostane ELISA Kit

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Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

You may be eligible to receive a free sample 8-Isoprostane EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Limit of detection: 80% B/B0: 2.7 pg/ml · Sensitivity: 50% B/B0: 10 pg/ml · The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF2α), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.4 Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.5 Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.5,6

1  Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF2α, in rabbit lung in situ. Am J Physiol 263 H660-H663 (1992). 2  Vacchiano, C.A., Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF2α, in pulmonary oxygen toxicity. J Appl Physiol 77 2912-2917 (1994). 3  Morrow, J.D., Frei, B., Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. N Engl J Med 332 1198-1203 (1995). 4  Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87 9383-9387 (1990). 5  Wang, Z., Ciabattoni, G., Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F2α excretion in man. J Pharmacol Exp Ther 275 94-100 (1995). 6  Reilly, M.P., Barry, P., Lawson, J.A., et al. Urinary 8-epi PGF2α: An index of oxidant stress in vivo. Fibrinolysis & Proteolysis 11 81-84 (1997).

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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.