See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

13,14-dihydro-15-keto Prostaglandin F2α EIA Kit

Cayman Chemical Item Number 516671

13,14-dihydro-15-keto Prostaglandin F2α ELISA Kit; PGFM EIA Kit

13,14-dihydro-15-keto Prostaglandin F2α EIA Kit

See analysis tools…

13,14-dihydro-15-keto Prostaglandin F2α EIA Kit Analysis Tools

Cayman offers a variety of analysis tools to assist you.

See more

Description

Limit of detection: 80% B/B0: 13 pg/ml · Sensitivity: 50% B/B0: 120 pg/ml · Prostaglandin F2α (PGF2α) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF2α was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Δ13-reductucase.1,2,3,4 Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α.5,6 The Cayman Chemical 13,14-dihydro-15-keto PGF2α Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2α within the range of 7.8-1,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 10 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2α in plasma, a common matrix for measurement of this PGF2α metabolite.5

1 Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu Rev Biochem 44 669-695 (1975).

2 Keirse, M.J.N.C., and Turnbull, A.C. Metabolism of prostaglandins within the pregnant uterus. Br J Obstet Gynaecol 82 887-893 (1975).

3 Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

4 Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

5 Del Vecchio, R.P., Maxey, K.M., and Lewis, G.S. A quantitative solid-phase enzymeimmunoassay for 13,14-dihydro-15-keto-prostaglandin F2α in plasma. Prostaglandins 43 321-330 (1992).

6 Meyer, H.H.D., Eisele, K., and Osaso, J. A biotin-streptavidin amplified enzyme immunoassay for 13,14-dihydro-15-keto-PGF2α. Prostaglandins 38 375-383 (1989).

Synonyms
  • 13,14-dihydro-15-keto Prostaglandin F2α ELISA Kit
  • PGFM EIA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
13,14-dihydro-15-keto Prostaglandin F2α 100%
13,14-dihydro-15-keto Prostaglandin E2 2.7%
15-keto Prostaglandin F2α 1.8%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin F1α <0.01%
2,3-dinor-6-keto Prostaglandin F1α <0.01%
6-keto Prostaglandin F1α <0.01%
Prostaglandin F2α <0.01%
8-iso Prostaglandin F2α <0.01%
8-iso-13,14-dihydro-15-keto Prostaglandin F2α <0.01%
8-iso-15-keto Prostaglandin F2α <0.01%
Prostaglandin F2β <0.01%
8-iso Prostaglandin F3α <0.01%
Thromboxane B2 <0.01%
Show all 15 Hide all but first 3

Background Reading

Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu Rev Biochem 44 669-695 (1975).

Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

Del Vecchio, R.P., Maxey, K.M., and Lewis, G.S. A quantitative solid-phase enzymeimmunoassay for 13,14-dihydro-15-keto-prostaglandin F2α in plasma. Prostaglandins 43 321-330 (1992).

Keirse, M.J.N.C., and Turnbull, A.C. Metabolism of prostaglandins within the pregnant uterus. Br J Obstet Gynaecol 82 887-893 (1975).

Meyer, H.H.D., Eisele, K., and Osaso, J. A biotin-streptavidin amplified enzyme immunoassay for 13,14-dihydro-15-keto-PGF2α. Prostaglandins 38 375-383 (1989).

Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

Show all 6 Hide all but first 3
516671-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
13,14-dihydro-15-keto Prostaglandin F2α AChE Tracer 500 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Antiserum 500 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Standard 1 ea
516671-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
13,14-dihydro-15-keto Prostaglandin F2α AChE Tracer 100 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Antiserum 100 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Standard 1 ea
516671-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
13,14-dihydro-15-keto Prostaglandin F2α AChE Tracer 100 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Antiserum 100 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Standard 1 ea
516671-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
13,14-dihydro-15-keto Prostaglandin F2α AChE Tracer 500 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Antiserum 500 dtn
13,14-dihydro-15-keto Prostaglandin F2α EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 13,14-dihydro-15-keto Prostaglandin F2α EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

13,14-dihydro-15-keto Prostaglandin F2α
Prostaglandin F2α EIA Kit
SPE Cartridges (C-18)
UltraPure Water
Show all 4 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?