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Latanoprost EIA Kit

Cayman Chemical Item Number 516811

Latanoprost ELISA Kit; PhXA 85 EIA Kit; 17-phenyl-13,14-dihydro trinor Prostaglandin F2α isopropyl ester EIA Kit; 17-phenyl-13,14-dihydro trinor Prostaglandin F2α EIA Kit

Latanoprost EIA Kit

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Latanoprost EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 15 pg/ml (free acid) and 43 pg/ml (ester) · Sensitivity: 50% B/B0: 58 pg/ml (free acid) and 200 pg/ml (ester) · Latanoprost is 13,14-dihydro-17-phenyl-18,19,20-trinor prostaglandin F2α isopropyl ester (13,14-dihydro-17-phenyl-18,19,20-trinor PGF2α isopropyl ester), an F-series prostaglandin analog which has been approved for use as an ocular hypotensive drug.1,2,3 Prostaglandin esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost (Latanoprost free acid (Lat-FA), or PhXA 85) is 200 times more potent than the isopropyl ester as an FP receptor ligand tested using the recombinant human FP receptor.2 Although the exact mechanism of the intraocular hypotensive effect of prostaglandins is unknown, it correlates very closely with the FP receptor ligand binding affinity of FP receptor agonists.4 The Cayman Latanoprost EIA is a sensitive detection method for measuring the free acid form of Latanoprost. It also detects the isopropyl ester form of Latanoprost, but with less sensitivity. The assay is most appropriate for use when only one of the two forms is present. Samples containing mixtures of both the isopropyl ester and free acid should be purified and the two compounds separated prior to EIA analysis.

1 Stjernschantz, J., and Resul, B. Phenyl substituted prostaglandin analogs for glaucoma treatment. Drugs Future 17 691-704 (1992).

2 Abramovitz, M., Adam, M., Boie, Y., et al. The utilization of recombinant prostanoid receptors to determine the affinities and selectivities of prostaglandins and related analogs. Biochim Biophys Acta 1483 285-293 (2000).

3 Camras, C.B., Alm, A., Watson, P., et al. Latanoprost, a prostaglandin analog, for glaucoma therapy. Efficacy and safety after 1 year of treatment in 198 patients. Ophthalmology 103 1916-1924 (1996).

4 Cravatt, B.F., Giang, D.K., Mayfield, S.P., et al. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature 384 83-87 (1996).

Synonyms
  • Latanoprost ELISA Kit
  • PhXA 85 EIA Kit
  • 17-phenyl-13,14-dihydro trinor Prostaglandin F2α isopropyl ester EIA Kit
  • 17-phenyl-13,14-dihydro trinor Prostaglandin F2α EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Latanoprost (free acid) 100%
5-trans Latanoprost 25.4%
Latanoprost 25%
15-epi Latanoprost 0.16%
15-keto Latanoprost 0.10%
Fluprostenol <0.01%
Fluprostenol isopropyl ester <0.01%
2,3-dinor Fluprostenol <0.01%
9-keto Fluprostenol <0.01%
11-keto Fluprostenol <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
6-keto Prostaglandin F1α <0.01%
Prostaglandin F2α <0.01%
5-trans Prostaglandin F2α <0.01%
tetranor-PGEM <0.01%
tetranor-PGFM <0.01%
Thromboxane B2 <0.01%
Unoprostone <0.01%
Unoprostone isopropyl ester <0.01%
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Background Reading

Powell, W.S. Reversed-phase high-pressure liquid chromatography of arachidonic acid metabolites formed by cyclooxygenase and lipoxygenases. Anal Biochem 148 59-69 (1985).

Stjernschantz, J., and Resul, B. Phenyl substituted prostaglandin analogs for glaucoma treatment. Drugs Future 17 691-704 (1992).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Cravatt, B.F., Giang, D.K., Mayfield, S.P., et al. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature 384 83-87 (1996).

Powell, W.S. Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using octadecylsilyl silica. Prostaglandins 20 947-957 (1980).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Basu, S., and Sjöquist, B. Development of a radioimmunoassay for latanoprost and its application in a long-term study in monkeys. Prostaglandins Leukot Essent Fatty Acids 55 427-432 (1996).

Abramovitz, M., Adam, M., Boie, Y., et al. The utilization of recombinant prostanoid receptors to determine the affinities and selectivities of prostaglandins and related analogs. Biochim Biophys Acta 1483 285-293 (2000).

Camras, C.B., Alm, A., Watson, P., et al. Latanoprost, a prostaglandin analog, for glaucoma therapy. Efficacy and safety after 1 year of treatment in 198 patients. Ophthalmology 103 1916-1924 (1996).

Goh, Y., and Kishino, J. Pharmacological characterization of prostaglandin-related ocular hypotensive agents. Jpn J Ophthamol 38 236-245 (1994).

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516811-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Latanoprost AChE Tracer 500 dtn
Latanoprost EIA Antiserum 500 dtn
Latanoprost (free acid) EIA Standard 1 ea
Latanoprost EIA Standard 1 ea
516811-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Latanoprost AChE Tracer 100 dtn
Latanoprost EIA Antiserum 100 dtn
Latanoprost (free acid) EIA Standard 1 ea
Latanoprost EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $246.00 $0.00
96 strip wells $246.00 $0.00
480 solid wells $923.00 $0.00
480 strip wells $923.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Latanoprost EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

17-phenyl trinor Prostaglandin F2α EIA Kit
17-trifluoromethylphenyl trinor Prostaglandin F2α ethyl amide
Latanoprost
Latanoprost (free acid)
Prostaglandin F2α EIA Kit
SPE Cartridges (C-18)
UltraPure Water
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