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11-dehydro Thromboxane B2 EIA Kit - Monoclonal New

Cayman Chemical Item Number 519510

11-dehydro TXB2 ELISA Kit

11-dehydro Thromboxane B2 EIA Kit - Monoclonal

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11-dehydro Thromboxane B2 EIA Kit - Monoclonal Analysis Tools

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Description

Limit of detection: 80% B/B0: 34 pg/ml · Sensitivity: 50% B/B0: 120 pg/ml · Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to ex vivo platelet activation or intra-renal production, and normal concentrations of circulating TXB2 are extremely low (1-2 pg/ml) and highly transient (t1/2 = 5-7 minutes). 11-dehydro TXB2 is a metabolite of TXB2 (not formed by platelets or the kidney) with a circulating half-life (t1/2) of 45 minutes. Its measurement in plasma or urine will give a time-integrated indication of TXA2 production and is recommended to estimate TXA2 levels to circumvent measurement complications associated with TXB2. Cayman’s 11-dehydro TXB2 EIA Kit is a competitive assay that can be used for quantification of 11-dehydro TXB2 in urine and other sample matrices. Normal plasma levels of 11-dehydro TXB2 (1-2 pg/ml) are below the detection limit of this assay (16 pg/ml), so plasma samples must be purified and concentrated prior to analysis. Urine samples should be diluted appropriately before assaying. There is evidence suggesting that 11-dehydro TXB2 can be formed non-enzymatically following platelet activation, so it is important to process plasma samples quickly to prevent artifactual formation of 11-dehydro TXB2. The EIA typically displays an IC50 (50% B/B0) value of 120 pg/ml and a detection limit (80% B/B0) of approximately 34 pg/ml.
Synonyms
  • 11-dehydro TXB2 ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
11-dehydro Thromboxane B2 100%
11-dehydro-2,3-dinor Thromboxane B2 330%
Prostaglandin D2 0.12%
2,3-dinor Thromboxane B2 0.10%
Thromboxane B2 0.08%
Arachidonic Acid <0.01%
Prostaglandin F2α <0.01%
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Background Reading

Wu, P., Fritzo, M., and Tai, H. Purification and characterization of an NAD+-dependent dehydrogenase that catalyzes the oxidation of thromboxane B2 at C-11 from porcine liver. Development and application of 11-dehydro-thromboxane B2 radioimmunoassay to enzyme assay. Biochim Biophys Acta 1036 55-63 (1990).

Ishibashi, M., Watanabe, K., Ishizaki, F., et al. Dimethylisopropylsilyl ether derivative in gas chromatography/mass spectrometry of 2,3-dinor-11-dehydrothromboxane B2. Biol Mass Spectrom 20 399-407 (1991).

Chiabrando, C., Rivoltella, L., Alberti, E., et al. Urinary excretion and origin of 11-dehydro-2,3-dinor-thromboxane B2 in man. Prostaglandins (1993).

Reinke, M. Monitoring thromboxane in body fluids: A specific ELISA for 11-dehydrothromboxane B2 using a monoclonal antibody. Am J Physiol 262 E658-E662 (1992).

Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu Rev Biochem 44 669-695 (1975).

Hamberg, M., Svensson, J., and Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 72 2994-2998 (1975).

Lawson, J.A., Patrono, C., Ciabattoni, G., et al. Long-lived enzymatic metabolites of thromboxane B2 in the human circulation. Anal Biochem 155 198-205 (1986).

Patrono, C., Ciabattoni, G., Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77 590-594 (1986).

Diczfalusy, U., Vesterqvist, O., Kase, B.F., et al. Peroxisomal chain-shortening of thromboxane B2: Evidence for impaired degradation of thromboxane B2 in Zellweger syndrome. J Lipid Res 34 1107-1113 (1993).

Fauler, J., Tsikas, D., Mayatepek, E., et al. Impaired degradation of prostaglandins and thromboxane in Zellweger syndrome. Pediatr Res 36(4) 449-455 (1994).

Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A2. Science 193 1135-1137 (1976).

Ciabattoni, G., Pugliese, F., Davi, G., et al. Fractional conversion of thromboxane B2 to urinary 11-dehydrothromboxane B2 in man. Biochim Biophys Acta 992 66-70 (1989).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

Catella, F., Healy, D., Lawson, J.A., et al. 11-dehydro Thromboxane B2: A quantitative index of thromboxane A2 formation in the human circulation. Proc Natl Acad Sci USA 83 5861-5865 (1986).

Djurup, R., Chiabrando, C., Jörres, A., et al. Rapid, direct enzyme immunoassay of 11-keto-thromboxane B2 in urine, validated by immunoaffinity/gas chromatography-mass spectrometry. Clin Chem 39 2470-2477 (1993).

Westlund, P., Fylling, A.C., Cederlund, E., et al. 11-hydroxythromboxane B2 dehydrogenase is identical to cytosolic aldehyde dehydrogenase. FEBS Lett 345 99-103 (1994).

Roberts, L.J., Sweetman, B.J., and Oates, J.A. Metabolism of thromboxane B2 in man. J Biol Chem 256(16) 8384-8393 (1981).

Salzman, P.M., Salmon, J.A., and Moncada, S. Prostacyclin and thromboxane A2 synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther 215 240-247 (1980).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Yasu, T., Oshima, S., Imanishi, M., et al. Effects of aspirin DL-lysine on thrombin generation in unstable angina pectoris. Am J Cardiol 71 1164-1168 (1993).

Tönshoff, B., Momper, R., Kühl, P.G., et al. Increased thromboxane biosynthesis in childhood hemolytic uremic syndrome. Kidney Int 37 1134-1141 (1990).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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519510-96 well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
11-dehydro Thromboxane B2 Monoclonal AChE Tracer 100 dtn
11-dehydro Thromboxane B2 Monoclonal Antibody 100 dtn
11-dehydro Thromboxane B2 Monoclonal EIA Standard 100 ng
11-dehydro Thromboxane B2 Monoclonal Assay Buffer Concentrate (10X) 2 × 10 mL
519510-480 well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
11-dehydro Thromboxane B2 Monoclonal AChE Tracer 500 dtn
11-dehydro Thromboxane B2 Monoclonal Antibody 500 dtn
11-dehydro Thromboxane B2 Monoclonal EIA Standard 100 ng
11-dehydro Thromboxane B2 Monoclonal Assay Buffer Concentrate (10X) 4 × 10 mL
519510-96 solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
11-dehydro Thromboxane B2 Monoclonal AChE Tracer 100 dtn
11-dehydro Thromboxane B2 Monoclonal Antibody 100 dtn
11-dehydro Thromboxane B2 Monoclonal EIA Standard 100 ng
11-dehydro Thromboxane B2 Monoclonal Assay Buffer Concentrate (10X) 2 × 10 mL
519510-480 solid
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
11-dehydro Thromboxane B2 Monoclonal AChE Tracer 500 dtn
11-dehydro Thromboxane B2 Monoclonal Antibody 500 dtn
11-dehydro Thromboxane B2 Monoclonal EIA Standard 100 ng
11-dehydro Thromboxane B2 Monoclonal Assay Buffer Concentrate (10X) 4 × 10 mL
Size Price Quantity Subtotal
96 solid wells $325.00 $0.00
96 strip wells $325.00 $0.00
480 solid wells $1,219.00 $0.00
480 strip wells $1,219.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 11-dehydro Thromboxane B2 EIA Kit - Monoclonal under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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UltraPure Water
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