15(S)-HETE EIA Kit

15(S)-HETE EIA Kit

Cayman Chemical Item Number 534721

15(S)-HETE ELISA Kit

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Pre-configured for analysis on MyAssays.com

Description

Limit of detection: 80% B/B₀: 170 pg/ml · Sensitivity: 50% B/B₀: 960 pg/ml · 15(S)-HETE is produced from arachidonic acid by the enzyme 15-lipoxygenase (15-LO). In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes.¹ 15(S)-HETE has been detected in high concentrations in nasal secretions and may contribute to allergic rhinitis.² 15(S)-HETE has anti-inflammatory properties, inhibiting carragheenan-induced arthritis and lowering leukotriene B₄ (LTB₄) concentrations in the synovial fluid of dogs.³ It may regulate T-lymphocytes by inhibiting 5- and 12-LOs.⁴ It is also a vasoconstrictor, constricting cerebral and coronary arteries of dogs in vitro and cerebral arteries of pigs in vivo.⁵ 15(S)-HETE may also play a role in cancer, inhibiting apoptosis by carcinosarcoma cells.⁶ Our 15(S)-HETE EIA is a competitive assay that permits the measurement of 15(S)-HETE within the range of 10-0.1 ng/ml, typically with a detection limit of 0.2 ng/ml. This assay is very specific for 15(S)-HETE, showing very low crossreactivity to other HETEs.

1 Hunter, J.A., Finkbeiner, W.E., Nadel, J.A., et al. Predominant generation of 15-lipoxygenase metabolites of arachidonic acid by epithelial cells from human trachea. Proc Natl Acad Sci USA 82 4633-4637 (1985).

2 Ramis, I., Roselló-Catafau, J., Bulbena, O., et al. 15-Hydroxyeicosatetraenoic acid as a major eicosanoid in nasal secretions: Assay by high-performance liquid chromatographic-radioimmunoassay and gas chromatographic-mass spectrometric procedures. J Chromatogr 496 416-422 (1989).

3 Fogh, K., Stender Hansen, E., Herlin, T., et al. 15-Hydroxy-eicosatetraenoic acid, (15-HETE) inhibits carragheenan-induced experimental arthritis and reduces synovial fluid leukotriene B₄ (LTB₄). Prostaglandins 37 213-228 (1989).

4 Bailey, J.M., Bryant, R.W., Low, C.E., et al. Regulation of T-lymphocyte mitogenesis by the leukocyte product 15-hydroxy-eicosatetraenoic acid (15-HETE). Cell Immunol 67 112-120 (1982).

5 Lovelady, G.K., Mirro, R., Armstead, W.M., et al. Effect of 15-HETE on cerebral arterioles of newborn pigs. Prostaglandins 36 507-513 (1988).

6 Tang, D.G., Chen, Y.Q., and Honn, K.V. Arachidonate lipoxygenases as essential regulators of cell survival and apoptosis. Proc Natl Acad Sci USA 93 5241-5246 (1996).

Synonyms

15(S)-HETE ELISA Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

15(S)-HETE	100%
15(S)-HETrE	3.03%
5(S),15(S)-DiHETE	2.87%
15(S)-HEPE	0.93%
8(S),15(S)-DiHETE	0.35%
(±)15-HEPE	0.21%
15(R)-HETE	0.08%
12(S)-HETE	0.04%
14,15-DiHETrE	0.03%
13(S)-HODE	0.02%
(±)14,15-EpETrE	<0.01%
5(R)-HETE	<0.01%
5(S)-HETE	<0.01%
8(S)-HETE	<0.01%
11(S)-HETE	<0.01%
12(R)-HETE	<0.01%
20-HETE	<0.01%
9(S)-HODE	<0.01%
13(R)-HODE	<0.01%
Leukotriene B4	<0.01%
Prostaglandin D2	<0.01%
Prostaglandin E2	<0.01%
6-keto Prostaglandin F1α	<0.01%
Prostaglandin F2α	<0.01%
Thromboxane B2	<0.01%

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Background Reading

Ramis, I., Roselló-Catafau, J., Bulbena, O., et al. 15-Hydroxyeicosatetraenoic acid as a major eicosanoid in nasal secretions: Assay by high-performance liquid chromatographic-radioimmunoassay and gas chromatographic-mass spectrometric procedures. J Chromatogr 496 416-422 (1989).

Bailey, J.M., Bryant, R.W., Low, C.E., et al. Regulation of T-lymphocyte mitogenesis by the leukocyte product 15-hydroxy-eicosatetraenoic acid (15-HETE). Cell Immunol 67 112-120 (1982).

Fogh, K., Stender Hansen, E., Herlin, T., et al. 15-Hydroxy-eicosatetraenoic acid, (15-HETE) inhibits carragheenan-induced experimental arthritis and reduces synovial fluid leukotriene B₄ (LTB₄). Prostaglandins 37 213-228 (1989).

Lovelady, G.K., Mirro, R., Armstead, W.M., et al. Effect of 15-HETE on cerebral arterioles of newborn pigs. Prostaglandins 36 507-513 (1988).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Hunter, J.A., Finkbeiner, W.E., Nadel, J.A., et al. Predominant generation of 15-lipoxygenase metabolites of arachidonic acid by epithelial cells from human trachea. Proc Natl Acad Sci USA 82 4633-4637 (1985).

Tang, D.G., Chen, Y.Q., and Honn, K.V. Arachidonate lipoxygenases as essential regulators of cell survival and apoptosis. Proc Natl Acad Sci USA 93 5241-5246 (1996).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

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534721-96WELL

15(S)-HETE EIA Kit

15(S)-HETE EIA Kit

15(S)-HETE EIA Kit Analysis Tools

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