COX Inhibitor Screening Assay Kit

Cayman Chemical Item Number 560131

COX Inhibitor Screening Assay Kit

See analysis tools…

Pre-configured for analysis on MyAssays.com

Description

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) enzymes contain both cyclooxygenase and peroxidase activities. COX catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins; the conversion of arachidonic acid to PGH₂. It is now well established that there are two distinct isoforms of COX. Cyclooxygenase-1 (COX-1) is constitutively expressed in variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, cyclooxygenase-2 (COX-2). COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions.¹ This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs. The COX Inhibitor Screening Assay directly measures PGF_2α produced by SnCl2 reduction of COX-derived PGH₂. The prostanoid product is quantified via enzyme immunoassay (EIA) using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, the Cayman COX assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.

1 Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

Stability

1 year

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Specificity

Prostaglandin E1	100%
Prostaglandin E2	100%
Prostaglandin F2α	100%
Prostaglandin F1α	100%
Prostaglandin F3α	51.3%
Prostaglandin E2 Ethanolamide	44.0%
6-keto Prostaglandin F1α	43.6%
8-iso Prostaglandin F2α	38.4%
8-iso Prostaglandin E2	28.5%
Prostaglandin D2	26.6%
8-iso-2,3-dinor Prostaglandin F1α	20.0%
Prostaglandin E3	9.5%
Thromboxane B2	5.0%
12(S)-HHTrE	0.25%
Leukotriene D4	0.2%
Arachidonic Acid	<0.01%
Leukotriene B4	<0.01%
Leukotriene C4	<0.01%
Leukotriene E4	<0.01%
Misoprostol	<0.01%
Misoprostol (free acid)	<0.01%
Prostaglandin A1	<0.01%
Prostaglandin A2	<0.01%
Prostaglandin A3	<0.01%
Prostaglandin B1	<0.01%
15-keto Prostaglandin E2	<0.01%
13,14-dihydro-15-keto Prostaglandin F2α	<0.01%

Show all 27 Hide all but first 3

Background Reading

Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

560131-96WELL

Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate

1 ea

Polysorbate 20

1 ea

Ellman's Reagent

3 × 100 dtn

EIA Buffer Concentrate (10X)

2 × 10 mL

Wash Buffer Concentrate (400X)

5 mL

Prostaglandin Screening AChE Tracer